体内和体外实验中马桑内酯对大鼠大脑皮质神经元白细胞介素-2受体表达的影响

THE EFFECT OF CORIARIA LACTONE ON THE EXPRESSION OF INTERLEUKIN-2 RECEPTOR OF NEURONS OF RAT CEREBRAL CORTEX IN VIVO AND IN VITRO EXPERIMENTS

为了探讨免疫调质与癫痫发病机制的关系，本文应用免疫细胞化学PAP法对体内和体外实验中马桑内酯（致痫剂）对大鼠大脑皮质种经元白细胞介素-2受体表达的影响进行了研究．在体内实验中，对照经大鼠大脑皮质仅见少量弱阳性白细胞介素-2受体免疫反应神经元，其免疫反应性定位于种经元膜上．大鼠一侧侧脑室注射马桑内酯诱发癫痫后，大脑皮质白细胞介素-2受体阳性神经元明显增多，免疫反应增强．在体外实验中，用马桑内酯温育培养大双皮质神经元24h，其白细胞介素2受体阳性神经元也比对照组增加了1．5倍，免疫染色加深．本实验结果提示，大脑皮质神经元上的白细胞介素-2受体可能参与癫痫的病理机制．

In order to explore the relationship between immunomodulator and the pathogenesis of epilepsy, the effect of Coriaria Lactone (epileptisant) on the expression of IL-2R of the neurons in rat cerebral cortex was studied by means of im-munocytocbemical PAP method in vivo and in vitro experiments. There are few and weak IL-2R immu noreaction-positive neurons in the rat cerebral cortex in vivo control group. After seizure induced by injection of Coriaria Lactone into the lateral ventricle, a lot and heavy IL-2R immu noreaction-positive neurons were showed in cerebral cortex. T be situ ation was similar invitro, IL-2R immunoreactive neurons increasd about 1. 5 times with dense immunostain in cultured neurons of rat cerebral cortex 24 h after treated by Coriaria Lactone. The results indicated that IL-2R in cerebral cortex might involve in the patho-genesis of epilepsy.