摘要
目的在乙型肝炎疫苗体外诱导B淋巴细胞产生HBs-Ab过程中,通过脂多糖和IL-2作为有效刺激物质,使用多种组合方法进行观察比较,了解影响抗体产生的体外诱导因素。方法用乙型肝炎疫苗刺激组(A组)、乙型肝炎疫苗+脂多糖刺激组(B组)、乙型肝炎疫苗+脂多糖+IL-2刺激组(C组)三组进行淋巴细胞培养和诱导,比较HBs-Ab产生情况,用酶联免疫吸附试验(ELISA)测定HBs-Ab含量。结果在脂多糖和IL-2的刺激诱导下,能有效促进B淋巴细胞产生HBs-Ab。结论实验证明脂多糖是激活人血B淋巴细胞的有效丝裂原,IL-2可促使活化B细胞增生及产生抗体,脂多糖和IL-2联合作用,可增强乙型肝炎疫苗诱导体液免疫水平。
[Objective] To investigate the effects of lipopolysaeeharide (LPS) and interleukin-2 (IL-2) on HBs- Ab production in B lymphocyte induced by hepatitis B vaccine in vitro. [Methods] B lymphoeytes were incubated and stimulated by hepatitis B vaccine (A group), hepatitis B vaccine plus LPS (B group), or hepatitis B vaccine plus LPS and IL-2 (C group) respectively. HBs-Ab was determined by enzyme linked immunosorbent assay (ELISA) in three groups. [Results] HBs-Ab was produced by B lymphocyte after the stimulation of LPS and IL-2. [Conclusions] LPS is the mitogen to activate human blood B lymphocyte. IL-2 stimulates the activated B cell to proliferate and to produce antibody. The combination of LPS and IL-2 can significantly stimulate the HBs-Ab production induced by hepatitis B vaccine in vitro.
出处
《中国医学工程》
2008年第2期81-83,共3页
China Medical Engineering
关键词
乙型肝炎疫苗
脂多糖(LPS)
IL-2
B细胞
hepatitis B vaccine
lipopolysaeeharide (LPS)
interleukin-2 01.,-2)
B lymphocyte