圈卷产色链霉菌分化关键基因——saw1的克隆及酶切分析

CLONING AND RESTRICTION ANALYSIS OF saw1——A KEY GENE FOR DIFFERENTIATION OF STREPTOMYCES ANSOCHROMOGENES

链霉菌是现代生物学研究中一种重要的微生物,它有两个突出的特征:其一,有无与伦比的合成次生代谢产物的能力,世界上所知数千种抗生素的70％由其产生。其二,有一个复杂的发育分化的生命周期,是微生物分化研究的一个最好的模式材料。链霉菌分化主要为形态分化和生理分化,两者彼此独立又相互关联,构成复杂的分化调控网络,研究分化基因的调控不但有重要的理论意义,而且可用于控制抗生素的生物合成,因此弄清合成途径的分子机制,也有潜在的应用价值。

sawl——a essential gene for sporulation of Streptomyces ansochromogeneswas cloned, 7 kb of DNA fragment in Streptomyces plasmid pIJ702 could recover the sporulation of sawl-deficient strain W51 which could not form spore with white phenotype of aerial hyphae. The recombinant plasmid was deleted by some restriction enzymes, the result showed that 2.5 kb DNA fragment in pIJ702 still has function to recover sporulation of W51. The sequencing and expression of sawl gene is earring out at present.