RAPD分析快速鉴定双歧杆菌

RAPID IDENTIFICATION OF BIFIDOBACTERIA BY RAPD

本文应用ＲＡＰＤ技术，选用１１种引物，以嗜酸乳杆菌为对照，对６种１３珠双歧杆菌菌株基因组ＤＮＡ进行ＰＣＲ扩增，分析其ＤＮＡ指纹图谱，并计算其相似性指数。结果表明，双歧杆菌和非双歧杆菌之间，其相似性指数有显著差异；选择合适的引物进行扩增，双歧杆菌不同种间和同种不同株间可表现不同的ＤＮＡ指纹图谱。本文还对ＲＡＰＤ技术应用于双歧杆菌分类鉴定的可能性进行讨论。

11 random primers were chosen to amplify the aegomic DNA from 13 strains belonged to 6 species of Bifidobacteria.Lactobacillus acidophillus and Escheichia coli were used as control.After analysing DNA fingerprinting pattems and calculating similarity index(Sl),we found that the difference of Sl between Bifidobacteria and non-Bifidobacteria was significant and that different species or strains Bifidobacteria showed different DNA fingerprinting patters using appropriate random primers.The possibility of using RAPD for Bifidobacteria idenfifcation qas discussed.