血纤蛋白粘附肽与低分子量单链尿激酶融合产物活性分析

Characterization of the Fusion Protein with a Fibrin adherent Peptide and scu PA 32K

人工合成了血纤蛋白粘附肽基因，构建了粘附肽与低分子量单链尿激酶ｃＤＮＡ的融合基因，在大肠杆菌中表达了融合基因。融合基因表达产物的抗原性和天然尿激酶相同，并具有尿激酶的溶纤活性和粘附肽的抗纤维蛋白单体聚合的功能。

The fibrin adherent peptide cDNA was chemically synthesized and fused with the scu PA 32K,the single chain urokinase of low molecular weight.The fused gene was expressed in E.coli .The results of activity assays showed that the fusion product remained the same antigenicity and the fibrinolytic activity as urokinase,obtaining the ability of inhibiting fibrin monomer polymerization in vitro.