TRPO-AOT 反胶团体系萃取牛血红蛋白的研究

Extraction of Bovine Hemoglobin with TRPO/AOT based Reversed Micelles

ＴＲＰＯＡＯＴ反胶团体系萃取牛血红蛋白的研究严勇朝刘会洲陈家镛（中国科学院化工冶金研究所分离科学与工程青年实验室北京１０００８０）反胶团是表面活性剂溶解在非极性溶剂中形成的、围绕一个“水核”的纳米级聚集体。液液反胶团萃取蛋白质技术，因对目标物质选...

It was found that solubilities of bovine haemoglobin(BHb)(MW=64 500,pI=6.7)in TRPO/AOT reversed micelles(RM)are higher than those in AOT RM and TRPO/isooctane,respectively.The distribution behavior of protein on pH for three systems are all in bell shape.The addition of neutral extractant trialkyl phosphine oxide(TRPO)to anionic surfactant sodium di 2 ethythexyl sulfosuccinate(AOT)RM results in a shift of the pH profile of BHb distribution behavior to high pH values.Especially,the back transfer of BHb from TRPO AOT RM is easer than that of BHb from AOT RM,The emulsion of the organic phase was found to influence the solubilization of protein unfavorably in RM.The determination of RM sizes by dynamic light scattering shows that the size of TRPO AOT RM is larger than that of AOT RM.