幽门螺杆菌细胞毒素相关基因A、空泡毒素基因A抗体与胃十二指肠疾病的关系

Relationship between cytotoxin-associated gene A as well as vacuolating cytotoxin gene A antibody of Helicobacter pylori and gastroduodenal diseases

目的研究不同幽门螺杆菌(Hp)菌株感染与胃十二指肠疾病发生的关系。方法采用蛋白芯片技术测定血清中Hp细胞毒素相关基因A(CagA)、空泡毒素基因A(VacA)抗体。结果消化性溃疡组和胃癌组CagA和VacA抗体阳性者显著高于慢性胃炎组(P<0.01),而CagA和VacA抗体阴性在慢性胃炎组显著高于溃疡组和胃癌组(P<0.01),溃疡组和胃癌组之间CagA和VacA抗体阳性和阴性率比较,差异无显著性(P>0.05)。结论含CagA基因和VacA基因的I型Hp可能是致病力强的高毒力株。

Objective To investigate the relationship between the different Strains of Helicobacter pylori（Hp） and gastroduodenal diseases. Methods Serum cytotoxin-associated gene A（CagA） and vacuolating cytotoxin gene A（VacA） antibody were tested by protein chip technique. Results The positive rates of both CagA and VacA antibody in the groups of peptic ulcer and gastric cancer were significantly higher than those of chronic gastritis（P 〈0.01）, the negative rates of both CagA and VacA antibody in the group of chronic gastritis were significantly higher than those of peptic ulcer and gastric cancer（P 〈0.01）, but the positive and negative rates of both CagA and VacA antibody were not significantly different between groups of peptic ulcer and gastric caneer（P 〉0.05）. Conclusion Type I Hp strains possessing CagA and VacA antibody might be highly virulent.