胃癌组织中IGF-Ⅰ表达与LVD的关系

Relationship Between the Expression of IGF-I and LVD in Gastric Carcinoma

目的:研究胃癌组织中胰岛素样生长因子Ⅰ表达与胃癌内淋巴管的关系及其意义。方法:运用RT-PCR的方法检测38例胃癌组织及15例正常胃组织中IGF-Ⅰ的表达情况,同时运用免疫组化检测其中的淋巴管及其密度。结果:IGF-ⅠmRNA在正常胃黏膜中有表达,但表达较少,而在胃癌组织中的表达比正常胃黏膜显著增高,正常组织中IGF-Ⅰ/GAPDH比值为(0.138±0.012),胃癌组织中IGF-Ⅰ/GAPDH比值为(0.372±0.020),P<0.01。经统计学分析IGF-Ⅰ表达与胃癌临床分期(r=0.588,P<0.01)、淋巴转移(r=0.493,P<0.05)正相关,与分化程度呈负相关(r=-0.462,P<0.01);但与患者性别、年龄无关,均无统计学意义,P>0.05。胃癌组织淋巴管密度(LVD)平均(9.52±0.44)个,正常组织淋巴管密度平均(4.29±0.66)个;淋巴管密度与淋巴转移(r=0.525,P<0.05)、临床分期(r=0.683,P<0.01)表达正相关,与分化程度负相关(r=-0.556,P<0.01),与IGF-Ⅰ的表达成显著正相关(r=0.732,P<0.001);与正常胃组织有显著差异,P<0.001;与性别、年龄无关,不具有统计学意义,P>0.05。结论:胃癌组织IGF-Ⅰ表达及淋巴管密度与胃癌临床分期呈正相关,与分化程度呈负相关,与胃癌淋巴转移成正相关,与性别、年龄无关;IGF-Ⅰ与肿瘤淋巴管密度正相关;IGF-Ⅰ可能促进LVD、加速肿瘤的淋巴转移。

Objective：To explore the relationship between the expression of IGF- Ⅰ and LVD in gastric carcinoma.Methods：S-P method and RT-PCR was applied in detecting carcinoma samples of 38 cases.Results：The expression of IGF- Ⅰ was positivily correlated with clinical stage （r=0.588, P 〈0.01） ,and lympha node metastasis （r=0.493, P 〈0.05） ,but was negatively correlated with pathologic differentiation （r=-0.462, P 〈0.01 ） .The expression of LVD was positivily correlated with clinical stage （r=0.683 ,P 〈0.01）, lympha node metastasis （r=0.525, P 〈0.05,and IGF- Ⅰ （r=0.732, P 〈0.001） ,but was negatively correlated with pathologic differentiation （r=-0.556, P 〈0.05）.Conclusion：In gastric cancer, the expression rate of IGF-Ⅰ and LVD was high. IGF-Ⅰ expression was positively correrated with LVD,and lympha node metastasis.It is presumed that IGF-Ⅰ can promote the metastasis of lyphma node in tumor by inducing LVD expression and the detection of IGF-Ⅰ in gastic cancer tissues will be significance for prediction of progonsis.