壳聚糖对大鼠Ⅲ度烧伤创面成纤维细胞生物学行为的影响

Changes of the biological behavior of dermal fibroblasts in Ⅲ skin burns wound in rats using chitosan

目的研究壳聚糖对烧伤创面成纤维细胞(FB)生物学行为的影响。方法Wistar大鼠分为1%壳聚糖组(W/V)、2%壳聚糖组(W/V)、4%壳聚糖组(W/V)、成纤维细胞生长因子(贝复剂)组(阳性对照组)、自然愈合组(模型组),制备成大鼠Ⅲ度烧伤模型,记录创面愈合时间,计算创面愈合率,用生化法测定各时相点羟脯氨酸(HOP),流式细胞术检测真皮组织中FB细胞周期,TUNEL法检测FB凋亡。结果4%壳聚糖组(W/V)各个时间点创面愈合率都明显高于自然愈合组(P<0.01);2%壳聚糖组、4%壳聚糖组创面愈合时间较自然愈合组缩短(P<0.05);2%壳聚糖组、4%壳聚糖组烧伤后7 d HOP就达最高值,且其值比自然愈合组最高值大;4%壳聚糖组进入S期的成纤维细胞数明显高于自然愈合组(P<0.05,P<0.01),创面凋亡细胞数量低于自然愈合组(P<0.05,P<0.01)。结论壳聚糖能增加烧伤创面修复细胞FB的增殖,凋亡减少,胶原合成增多,可能是壳聚糖促进大鼠Ⅲ度烧伤创面愈合的原因之一。

Aim To study the changes of the biological behavior of dermal fibroblasts in Ⅲ skin burns wound in rats using chitosan. Methods Wistar rats were randomly divided into 5 groups as follows：1% chitosan （W/V） group, 2% chitosan （W/V） group, 4% chitosan （W/V） group, bFGF （ basic fibroblast growth factor） group and the control group. Rats were made for Ill skin bums. The wound healing time was recorded, and the wound healing rate was calculated. Then the cell cycle and apoptotic dermal fibroblasts were determined and the amount of Hydroxyproline （HOP） in the skin tissue was analyzed. Results The wound healing rate of 4% chitosan （W/V） group was higher and the wound healing time of 4% chitosan （W/V） group was shorter than that of the control group. On the 7th , 14th day post-injury, the content of protein of 4% chitosan （W/V） group was higher than that of the control group. The content of HOP of 2% and 4% chitosan （W/V） group was highest on the 7th day post-injury. Compared with that in control group, the percentage of cells of S stage in 4% chitosan （W/V） group was aboundant,and was reduced in apoptotic dermal fibroblasts. Conclusion The changes of cellular biological behaviors might be one of the mechanisms of that Chitosan could promote the wound healing of m skin burns in rats significantly.