导管致小鼠颈总动脉血管损伤病变的实验研究

Detection of catheter induced carotid artery injury in mice

目的应用小鼠颈总动脉血管内膜损伤模型,观察并在体显示导管所致血管损伤病变情况。方法通过在小鼠左颈总动脉中插入并旋转导管导线造成血管损伤。于伤后1、3、4周行血管组织切片,HE染色对比观察损伤后血管内膜及中层等厚度及面积变化。并行免疫组化染色观察血管损伤后αVβ3分子表达及Ki67阳性细胞数的变化。最后在不同血管损伤程度动物,注射可与αVβ3分子特异性结合的111In-RP478,2.5h后分离出损伤及对照血管,检测其放射自显影强度。结果颈总动脉损伤后,血管内膜增生,出现大量新生内膜。新生内膜中有大量αVβ3表达。血管中层明显变厚。在血管中层及新生内膜中有大量Ki67阳性细胞。放射自显影发现,损伤侧显影强度明显高于对照侧,对比系数与损伤程度成正比。结论以αVβ3为靶分子的同位素显影可能为检测血管病变提供帮助。

Abstract： Objective To observe and detect catheter induced blood vessel injury by establishing the mouse model. Methods Catheter guidewire was induce vascular injury in mice. After 1, 3,4 weeks, the injured vessel was sectioned, the pathological changes of vessel was checked by HE stain, and the expression of αVβ3 and Ki67 positive cells in the sections were detected by immunohistochemical stain.^[1] In-RP478, a specific small ligand for αVβ3 was injected into injured mice to separate the injured vessel from the non-injured vessel 2.5 h later, and their autoradiography was measured. Results Neointima in the injured left common carotid was shown up and the media thickened significantly after injury. Expression of αVβ3, mainly in neointima, were increased after injury and a lot of Ki67 positive cells were found in neointima and media of the injured vessel. Intensity of injured autography was much higher than that of control. The intensity ratio of injured left common carotid artery to non-injured was in positive relationship with the degree of injury. Conclusion Catheter could induce vascular injury, and RP478 could be a good tracer for the injured vessel.