强直性脊柱炎活动期VEGF和TNF-α在棘间韧带组织中mRNA表达水平的检测

Expressions of VEGF and TNF-α in interspinous ligament of patients with ankylosing spondylitis in active stage

目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和肿瘤坏死因子(tumor nerosis factor,TNF-α)在强直性脊柱炎(ankylosing spondylitis,AS)活动期患者棘间韧带的病理性表达水平。方法通过原位杂交技术检测活动期AS患者与对照组棘间韧带组织中TNF-αmRNA、VEGF mRNA的表达,用图像分析系统比较它们的表达差异,并与疾病的活动指数、CRP进行相关性分析。结果在活动期AS患者棘间韧带组织中TNF-α mRNA、VEGF mRNA呈阳性表达,而对照组棘间韧带组织中TNF-α mRNA、VEGF mRNA呈阴性表达,阳性细胞计数和图像灰度值比较有统计学差异(P<0.01)。VEGF mRNA、TNF-α mRNA的表达与AS疾病活动指数(BASDAI)和CRP显著相关(r=0.53,r=0.42,P<0.05)。结论VEGF可能是强直性脊柱炎发病过程中的重要因子之一,TNF-α作为1个核心的调控因子,在强直性脊柱炎发病机制中也起着重要作用。

Objective To detect the pathological expressions of TNF-α and VEGF in interspinous ligament of patients with ankylosing spondylitis（AS） in active stage. Methods The expressions of TNF-α mRNA and VEGF mRNA in the interspinous ligament of patient with AS group and control group were detected by hybridization in situ. Their different results were compared by image analysis system and the correlation between TNF-α mRNA, VEGF mRNA and both AS disease active index （DAI） and C reactive protein （CRP） was analysed respectively. Results The expressions of TNF-α mRNA and VEGF mRNA in the interspinous ligament of patients with active AS were positive, but those of the control group were negtive. Statistical differences existed in the positive cell numbers and in image gray scale value of AS group （P 〈0. 01 ）. Significant correlation was observed between the expression of VEGF, TNF-α and CRP, BASDAI （P 〈0. 05）. Conclusion VEGF are the important factors in the pathological process in patients with active AS. TNF-α, a core reguahing factor, also plays an significant role in the pathogenesis of AS.