细胞培养与PCR法检测生殖器疱疹病毒的对比研究

Comparison of virus isolation and PCR on detection virus infection

目的：比较不同方法检测生殖器疱疹病毒感染的效率。方法：采用病毒培养和PCR法检测疑为生殖器疱疹病毒感染者标本60例，并用PCR作病毒分型。结果：根据病史及临床表现确诊的生殖器疱疹患者标本60例中，病毒培养法阳性36例，阳性牢60％（36／60）；PCR检测单纯疱疹病毒（HSV）阳性45例，阳性率75％（45／60），和病毒培养相比，差异非常显著（χ^2=26．76，P〈0．01）；PCR分型结果显示阳性标本均为单纯疱疹病毒Ⅱ型（HSV-2）。水疱和脓疱标本的病毒培养和PCR检测阳性率均较高（80．0％～93．3％），糜烂、结痂、斑丘疹标本检测中PCR阳性率（20．0％～66．7％）高于病毒培养（0～33．3％）。结论：对疑为生殖器疱疹患者作病原学诊断，采集水疱、脓疱标本进行病毒分离或PCR检测较佳。

Objective：In order to compare the virus isolation with PCR assay in detection virus infection. Methods：Sixty samples were collected from patients and performed with PCR detection and virus isolation using Veto cells. The virus typing was also conducted by PCR. Results：The results showed that 45 out of 60 samples （75%） were positive in PCR detection, which was significantly higher than that of virus isolation （60% , χ^2 = 26.76, P 〈 0.01 ）. All positive samples exhibited containing HSV-2 DNA in PCR typing with HSV type-specific primers and suggested that HSV-2 is the chief etiological factor of human. Conclusion：The blister and pustule samples were more suitable for detection of HSV infection with virus isolation and PCR assay in clinical than dissipated, scabed and maculopapule ones.