深山含笑和乐昌含笑叶片挥发油的成分分析及性能测定

Compositions and Properties of Volatile Oils from Leaves of Michelia maudiae Dunn and Michelia chapensis Dandy

采用超临界二氧化碳萃取法提取深山含笑和乐昌含笑叶片挥发油,应用GC-MS技术测定挥发油的成分并对其DPPH自由基清除能力和还原能力等抗氧化指标进行测定。结果表明:深山含笑萜类为35.35%,酯类为15.12%,醇类为9.09%,其余37.02%为萜烯类含氧衍生物。乐昌含笑萜类为28.49%,酯类为11.12%,醇类为1.94%,其余54.55%为萜烯类含氧衍生物。两种含笑叶片挥发油质量浓度为50mg/L时DPPH自由基清除能力达到饱和,且挥发油质量浓度越大还原能力越强。通过与常用抗氧化剂进行比较,发现两种含笑叶片挥发油的抗氧化能力均强于植酸PA没食子酸丙酯PG和抗坏血酸Vc。

The volatile oils from Michelia maudiae Dunn and Michelia chapensis Dandy leaves were extracted by supercritical carbon dioxide. Their compositions were detected by GC-MS , meanwhile antioxidation activities such as scavenging rate on DPPH free radical and reducing power were determined. Results show that the constitutes of M. maudiae were terpene 35.35 %, ester 15.12 % and alcohol 9.09 %. The rest 37.02 % were terpene oxydirivatives, the constitutes of M. chapensis were terpene 28.49 % , ester 11.12 % and alcohol 1.94 % . The rest 54.55 % are terpene oxydirivatives. When the concentrations of the two volatile oils were 50 mg/L, the scavenging rate on DPPH free radical attained to saturation. The concentration of volatile oil is higher, the reducing capability is stronger. Compared with common antioxidations, the antioxidant capacity of two species Michelia were stronger than that of PA, PG and Vc.