Effect of cefodizime on cytokines expression in mouse neutrophils and its related mechanism

Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results： Cefodizime increased the neutrophils production of TNF-α, IL-β3 and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion： Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.

Objective: To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae (Kle. p) treatment. Methods: We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 (TLR4) and the inhibitor factor of κBα (I-κBα) expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay (EMSA). Results: Cefodizime increased the neutrophils production of TNF-α, IL-1β and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion: Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.