摘要
目的:研究成纤维细胞激活蛋白((fibroblast activation protein alpha,FAPA)mRNA在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)及配对正常口腔黏膜组织中的表达。方法:采用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔黏膜中FAPAmRNA的表达;所得数据用SAS6.12软件包进行χ2检验和t检验。结果:RT-PCR检测结果表明,FAPAmRNA在30例OSCC和配对正常黏膜的阳性率分别为83%(25/30)和40%(12/30)(P<0.001);总表达水平分别为3.58±0.46和1.27±0.21,上调2.82倍(P<0.0001)。结论:在OSCC的癌变过程中,FAPA基因过表达,可能是OSCC的一个靶基因;FAPA在OSCC诊断中具有应用价值。
PURPOSE: To investigate the expression of fibroblast activation protein alpha(FAPA) gene in oral squamous cell carcinoma(OSCC) and paired normal tissue. METHODS: Differential FAPA gene expression paired normal and cancerous tissues from 30 OSCC patients were detected by reverse transcription-PCR (RT-PCR). The data was analyzed with SAS6.12 software for Chi-square test and t Student's test. RESULTS: RT-PCR results demonstrated that the positive rate of FAPA mRNA in 30 cancerous tissues and paired normal samples were 83%(25/30) and 40%(12/30), respectively (P〈0.001); the relative expression level of FAPA mRNA in 30 cancerous tissues and paired normal samples were 3.58±0.46 and 1.27±0.21, respectively (P〈0.0001), up-regulated 2.82-fold in OSCC cancerous tissues. CONCLUSIONS: FAPA mRNA overexpression may play an important role in OSCC carcinogenesis and could be a potential target of OSCC therapy.
出处
《中国口腔颌面外科杂志》
CAS
2008年第4期270-273,共4页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金重点项目(30330580)
上海市重点(优势)学科建设项目(Y0203)~~