口腔鳞癌与正常黏膜中FAPA基因mRNA的表达

Expression of FAPA gene in oral squamous cell carcinoma and paired normal tissue

目的:研究成纤维细胞激活蛋白((fibroblast activation protein alpha,FAPA)mRNA在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)及配对正常口腔黏膜组织中的表达。方法:采用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔黏膜中FAPAmRNA的表达;所得数据用SAS6.12软件包进行χ2检验和t检验。结果:RT-PCR检测结果表明,FAPAmRNA在30例OSCC和配对正常黏膜的阳性率分别为83%(25/30)和40%(12/30)(P<0.001);总表达水平分别为3.58±0.46和1.27±0.21,上调2.82倍(P<0.0001)。结论:在OSCC的癌变过程中,FAPA基因过表达,可能是OSCC的一个靶基因;FAPA在OSCC诊断中具有应用价值。

PURPOSE： To investigate the expression of fibroblast activation protein alpha（FAPA） gene in oral squamous cell carcinoma（OSCC） and paired normal tissue. METHODS： Differential FAPA gene expression paired normal and cancerous tissues from 30 OSCC patients were detected by reverse transcription-PCR （RT-PCR）. The data was analyzed with SAS6.12 software for Chi-square test and t Student＇s test. RESULTS： RT-PCR results demonstrated that the positive rate of FAPA mRNA in 30 cancerous tissues and paired normal samples were 83%（25/30） and 40%（12/30）, respectively （P〈0.001）; the relative expression level of FAPA mRNA in 30 cancerous tissues and paired normal samples were 3.58±0.46 and 1.27±0.21, respectively （P〈0.0001）, up-regulated 2.82-fold in OSCC cancerous tissues. CONCLUSIONS： FAPA mRNA overexpression may play an important role in OSCC carcinogenesis and could be a potential target of OSCC therapy.