槲寄生碱对人腺样囊性癌细胞的抑制作用

Inhibitory effect of mistletoe alkali on adenoid cystic carcinoma cells

目的:研究槲寄生碱对人腺样囊性癌(ACC)细胞生长的抑制作用。方法:以5-氟尿嘧啶(5-FU)作用组为阳性对照组,以不加药组为阴性对照组,实验组为0.625、1.250、2.500和5.000mg·L-1槲寄生组。采用CCK-8试剂盒测定槲寄生碱对ACC细胞的抑制作用,通过绘制细胞生长曲线观察药物作用后细胞的生长情况及状态。利用PCNA免疫化学染色观察槲寄生碱对ACC细胞增殖的影响。结果:槲寄生碱可抑制ACC细胞的生长,其IC50值为2.24mg·L-1;生长曲线显示,药物作用后的ACC细胞生长较对照组缓慢;形态学观察显示药物作用后的ACC细胞逐渐变为圆形,附壁性减弱;药物作用后的ACC细胞PCNA的表达率明显弱于对照组(P<0.001)。结论:槲寄生碱可以抑制ACC细胞生长、增殖,有望成为临床治疗ACC的辅助药物。

Objective To study the inhibitory effect of mistletoe alkali on human adenoid cystic carcinoma （ACC） cells in vitro. Methods The inhibitory effect on the ACC cells treated by mistletoe alkali was evaluated by CCK-8 assay. The ACC cells treated by 5-FU were used as positive control group and the ACC cells without any treatment as negative control group, meanwhile different doses of mistletoe alkali （ 0. 625, 1. 250, 2. 500 and 5. 000 mg · L^-1 ） were used as experimental groups. The growth of ACC cells after treatment was assessed by cell growth curve, and the cell proliferation was estimated by PCNA immunohistochemistry staining. Results Mistletoe alkali inhibited the growth of ACC cells, the value of IC50 was 2.24 mg · L^-1 . After treatment, the growth of ACC cells slowed, and the cells became round, and the expression of PCNA was weaker significantly than that in positive control （P〈0. 001）. Conclusion Mistletoe alkali can inhibit the growth and proliferation of ACC cells obviously in vitro. It might be an ancillary drug to cure ACC.