氯化消毒饮水中有机提取物对雄性小鼠生殖系统的影响

Effects of organic extractions from drinking water chlorinated disinfection on genital system in male mice

目的:研究氯化消毒饮水中有机提取物对雄性小鼠生殖系统的影响,为制定安全饮水的卫生决策提供依据。方法:以XAD-16大孔树脂富集枯水期氯化消毒饮水中的有机提取物。选雄性小鼠40只,随机分为4组,每组10只,分别为阴性对照组(给予植物油)及自来水有机提取物高(500mg·kg-1,相当于50L水中有机提取物)、中(250mg·kg-1)、低(125mg·kg-1)剂量组。每日经口染毒1次,连续15d,测定睾丸和血清中的睾酮含量、睾丸组织中乳酸脱氢酶(LDH)和6-磷酸葡萄糖脱氢酶(G-6-PD)的活性。结果:各染毒组血清睾酮及睾丸睾酮含量均明显低于对照组(P<0.05);中、高剂量组的LDH活性均明显低于对照组及低剂量组(P<0.05);高剂量组G-6-PD活性明显高于对照组及其他各染毒组(P<0.05)。结论:在本实验条件下,氯化消毒饮水中有机提取物可以使雄性小鼠的血清及睾丸组织中的睾酮含量减少,睾丸生殖酶活性下降,提示该提取物对雄性小鼠生殖系统可产生毒性作用。

Objective To study the effects of organic extractions from drinking water chlorinated disinfection on the genital system in male mice and provide evidence of safety drinking water for formulating healthy policy. Methods The organic extractions from drinking water were enriched by XAD-16 macroporous resin. 40 male mice were divided into 4 groups： control （plant oil ＋ DMSO）, high dose organic extractions （500 mg·kg^-1 , about equal to 50 L water）, middle dose （250 mg·kg^-1）, low dose （125 mg·kg^-1） . The organic extractions were administered 1 time every day through mouth for 15 d. The testosterone levels in blood serum and testis, the activities of LDH and G-6-PD were measured. Results The testosterone levels in serum and testis in every treatment group were lower than that in control group （P〈0.05）. In middle and high doses groups, the activities of LDH were lower than those in low dose group and control group obviously （P〈0.05）. In high dose group, the activity of G-6-PD was higher than those in all the other groups obviously （P 〈 0.05）. Conclusion Organic extractions can decrease the activities of G-6-PD and LDH, reduce the testosterone levels in serum and testis, and damage the genital system in male mice.