GPRA基因多态性与儿童支气管哮喘的关联性

Association between GPRA gene polymorphism and bronchial asthma in children

目的:探讨位于7号染色体(q15-14)的GPRA基因多态性与中国北方汉族儿童支气管哮喘的关系。方法:采用PCR-RFLP方法检测118例儿童支气管哮喘患者和123名健康对照儿童GPRA基因rs324960和rs324389位点的单核苷酸多态性(SNP)。结果:rs324960位点和rs324389位点PCR扩增目的片段分别为300bp和359bp,经酶切后都呈现CC、CT、TT3种基因型。2位点的3种基因型分布符合Hardy-Weinberg平衡定律。rs324960位点在两组人群中基因型频数分布(χ2=1.73,P>0.05)和等位基因频数分布(χ2=1.43,P>0.05)差异均无显著性;rs324389位点基因型频数分布(χ2=0.27,P>0.05)和等位基因频数分布(χ2=0.07,P>0.05)差异无显著性。结论:GPRA基因rs324960和rs324389位点的SNP可能与儿童支气管哮喘的发生无关联。

Objective To investigate the genetic association between GPRA gene located in chromosome 7 with susceptibility and bronchial asthma in Chinese Han children. Methods The techniques of PCR and restriction fragment polymorphism （RFLP） were used to examine the single nucleotide polymorphism （SNP） of GPRA gene in 118 children with bronchial asthma （ case group ） and 123 healthy individuals （control group）. Results The fragments of rs324960 and rs324389 were 300 bp and 359 bp separately, and three genotypes （CC, CT, TT） were found after digestion. The distributions of genotype frequencies of rs324960 and rs324389 were not deviated from Hardy-Weinberg equilibrium. There were no remarkable difference of the frequency distributions of genotypes of rs324960 between cases and controls （χ^2 = 1.73, P〉 0.05）, and the frequency distributions of allele had no markedly difference between them （χ^2 =1.43, P〉0.05）, and neither of rs324389 with the frequency distributions of genotypes （χ^2 =0.27, P〉0.05） and allele （χ^2 =0.07, P〉0.05）. Conclusion The SNPs of rs324960 and rs324389 of GPRA gene may not be associated with asthma in children.