TGF-β1和p21WAF1在中耳胆脂瘤中的表达及其意义

Expressions of TGF-β1 and p21WAF1 in middle ear cholesteatoma and their significances

目的:探讨TGF-β1和p21WAF1在中耳胆脂瘤中的表达,了解两者在胆脂瘤发病中的作用。方法:应用免疫组化技术检测20例中耳胆脂瘤组和10例正常对照组中TGF-β1和p21WAF1的表达。结果:①p21WAF1定位于细胞核,呈棕黄色颗粒状;TGF-β1主要胞浆着色,呈棕黄色颗粒状。②中耳胆脂瘤组p21WAF1阳性表达率65%,分布于上皮的全层,以靠近基底层的棘细胞层和颗粒层显著,阳性指数(LI)均值为28.9%±6.7%;而正常对照组未见p21WAF1阳性表达。p21WAF1在中耳胆脂瘤上皮中的表达高于对照组(P<0.05)。③中耳胆脂瘤组TGF-β1阳性表达率90%,位于上皮全层,LI为32.3%±5.7%;在正常外耳道皮肤中阳性表达率为50%,LI为13.3%±4.9%,两组比较差异有显著性(P<0.05)。④在中耳胆脂瘤组p21WAF1与TGF-β1表达LI均值比较差异无显著性(P>0.05),p21WAF1表达LI均值与TGF-β1表达LI均值呈正相关关系(r=0.913,P<0.01)。结论:TGF-β1和p21WAF1蛋白促进胆脂瘤发生发展,并具有相关性。

Objective To study the expressions of TGF-β1 and p21WAF1 in middle ear cholesteatoma and discuses their effects on cholesteatoma. Methods The expressions of TGF-β1 and p21WAF1 were determined by immunohistochemical S-P technology and computer image analysis in 20 specimens of cholesteatoma epithelium and 10 specimens of normal external canal skin. Results （1） p21WAF1 and TGF-β1 could be detected in middle ear cholesteatoma and in normal ear canal skin. p21WAF1 was located at karyon, taking on yellow-brown pellet; TGF-β1 was located at cytoplasmic, taking on yellow-brown pellet. （2）The p21WAF1 positive rate in middle ear cholesteatoma was 65% and there were almost no positive cells in normal external ear canal skin. p21WAF1 positive cells were detected in each layers, its LI was 28.9%±6.7%. Compared with normal ear canal skin, the expression level of p21WAF1 in middle ear cholesteatoma epithelium was higher than that in normal ear canal skin （P〈0.05）. （3） The TGF-β1 positive rate was 90% in middle cholesteatoma samples and 50% in normal ear canal skin. The positive cells were mostly in spine layer and granular layer in normal ear canal skin, low intensive, lightbrown, and its LI was 13.3±4.9 % in middle ear cholsteatoma epithelium, the positive cells scattered among all layers of the epithelium, and stained strongly in spine layer, high intensive, strong-brown, and its LI was 32.3%± 5.7%, there was obvious difference （P〈 0.05）. （4） In cholesteatoma epithelium samples, the LI of p21WAF1 and TGF-β1 didn t have significant difference. But in cholesteatomatous tissues there was positivecorrelation between the LI average of p21WAF1 and the LI average of TGF-β1 （r = 0. 913, P 〈 0. 001） . Conclusion TGF-β1 and p21WAF1 can promote the occurrence and development of middle ear cholesteatoma, and they have positive correlation.