人激肽释放酶10在卵巢癌组织中的表达及其意义

Expression of hK10 in ovarian cancer tissue and its significances

目的:研究人激肽释放酶10(hK10)在卵巢癌组织中的表达,探讨hK10基因在卵巢癌早期诊断研究中的意义。方法:①应用4097靶基因点基因芯片对5例卵巢浆液性囊腺癌及5例正常卵巢组织进行基因表达谱分析,筛查差异表达基因。②应用RT-PCR检测32例卵巢浆液性囊腺癌组织、20例卵巢浆液性囊腺瘤组织及16例正常卵巢组织中hK10表达并对比分析。结果:①基因芯片筛查出差异表达基因58个,其中表达增高(上调趋势)基因30个,表达降低(下调趋势)基因28个,hK10基因为表达上调基因(Cy5/Cy3=3.031)。②hK10基因在正常卵巢及卵巢瘤组织中有弱表达(0.023±0015和0.023±0.045),在卵巢癌组织中表达明显增强(0.106±0.045),hK10基因在卵巢癌组的表达明显高于正常卵巢组及卵巢瘤组(P<0.01)。结论:基因芯片能够用于筛查卵巢癌差异表达基因;hK10基因在卵巢癌组织表达明显增强,有望成为卵巢癌的肿瘤标志物。

Objective To study the expression of hK10 in ovarian cancer tissue and the significances of hk10 gene in the early diagonosis of ovarian cancer. Methods （1）cDNA microarray containing 4 097 genes was used to analyze gene expression patterns in samples from 5 cases of human ovarian serous cystadenocarinoma and 5 cases of normal ovarian tissues; （2）RT-PCR method was used to investigate the expressions of hK10 mRNA in samples of 32 serous cystadenocarinoma, 20 serous cystadenoma and 16 normal ovarian tissues, Results （1） 58 genes of differential expression were found in ovarian cancer tissue by cDNA microarray. The expression of 30 genes increased （upregulated）, the expression of 28 genes decreased （down-regulated）. hk10 was the up-regulated gene （Cy5/Cy3= 3. 031）. （2）The expression levels of hK10 gene in normal ovarian tissues and benign ovarian tumor tissues were lower, but higher in ovarian cancer tissues. The expression level of hK10 was 0. 023 ± 0. 015 in normal ovarian tissues, 0. 032±0. 045 in benign ovarian tumor tissues and 0. 106±0. 045 in ovarian cancer tissues. The expression level of hK10 gene in ovarian cancer tissues was significantly higher than those in normal ovarian and benign ovarian tumor （P〈0.01）. Conclusion cDNA microarray could be used to screen differential expression gene in ovarian cancer; the expression level of hK10 gene is increased in ovarian cancer tissues, it may be a new tumor marker for ovarian cancer.