大肠癌细胞辐射敏感性的蛋白组学研究

The research of proteomics on the radiosensitivity of colorectal carcinoma cells

背景与目的:大肠癌细胞系SW480与LoVo具有不同的辐射敏感性,我们运用蛋白组学技术筛选大肠癌细胞辐射敏感相关的蛋白。方法:采用SELDI-TOF蛋白芯片技术分别对经0Gy、2Gy、4Gy和6Gy照射后24h的SW480与LoVo细胞进行蛋白质谱分析。结果:LoVo细胞在平均质荷比分别为2478.24、4521.30、5383.82和8454.03的蛋白质峰值随辐射剂量增加而逐渐下降;SW480细胞在平均质荷比分别为5655.29、7259.53的蛋白质峰值随辐射剂量增加逐渐升高,在平均质荷比分别为15829.02,17859.47的蛋白质峰值随辐射剂量增加呈梯度下降。结论:大肠癌细胞辐射敏感性的预测可以通过蛋白质水平来体现。

Background and purpose： Two human colorectal cancer cell lines LoVo and SW480, have different degrees of radiosensitivity, We applied proteomics technology to the screening of radiosensitivity-related proteins in human colorectal cancer cells. Methods： The human colorectal cancer cell lines, LoVo and SW480, were received a succession of 0, 2, 4, and 6 Gy X-ray radiation. 24 hours after radiation, the protein spectra of each cell line were measured by SELDI-TOF protein chip. Results： In the LoVo cell line, 4 protein peaks showed fall-off changes in a dose-dependent manner, the average m/z values of which were 2 478.24,4 521.30,5 383.82 and 8 454.03 respectively; in the SW480 cell line, 2 protein peaks showed step-up change with dose-dependent manner, the average m/z values of which were 5 655.29 and 7 259.53 respectively; 2 protein peaks showed fall-off change with dose-dependent manner, the average m/z values of which were respectively 15 829.02 and 17 859.47. Conclusions： Radiosensitivity in colorectal cancer cells can be predicted at protein level.