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基因芯片分析Wnt刺激后小鼠多潜能分化干细胞C3H10T1／2的差异基因表达 被引量：2

Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection:a result with cDNA microarray assay
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摘要 目的探讨Wnt信号传导系统对诱导干细胞向神经系统分化的基因表达的影响。方法采用最新的含有22000多个已知基因及EST克隆的小鼠基因芯片U74Av2,检测了Wnt刺激后的小鼠多潜能分化干细胞C3H10T1/2中目标基因表达的变化,并进行实时定量PCR,验证了基因芯片结果。结果Wnt刺激后小鼠多潜能分化干细胞C3H10T1/2中Wnt信号传导系统各主要组成基因表达有不同程度增加;对干细胞向神经系统发育有潜在调节作用的基因表达也有增加或减少。结论表达谱芯片分析成功筛选出Wnt刺激引起表达改变且可能与神经发生有关的基因。 Objective To explore the effect of Wnt signaling pathway on neural differentiation of stem cells. Methods Multiple target genes were analyzed in mouse stem cells C3H10T1/2 by mouse gene chip U74Av2, a novel microarray possessed over 22 000 target genes. Real time PCR was applied for the validation of microarray result, Results The expression of major components of Wnt signaling pathway was increased to various extent. The expression of some potential genes related to neurogenesis increased, the others decreased. Conclusion With cDNA microarray technology, specific genes related to neurogenesis were screened successfully, the expression of which were regulated by Wnt in mouse stem cells C3H10T1/2.
作者 朱丹 谢鹏 康权 黄文 曾志磊 牟君 魏有东 陈晓 彭丹 刘庆军
机构地区 重庆医科大学附属第一医院神经内科 重庆医科大学附属儿童医院小儿外科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2008年第17期1604-1606,共3页 Journal of Third Military Medical University
基金 国家自然科学基金(30370500)~~
关键词 Wnt信号传导系统 神经系统发育 基因芯片 干细胞 Wnt signaling pathway neurogenesis microarray stem cell
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学] R394-33 [医药卫生—医学遗传学]
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参考文献14

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同被引文献28

  • 1师伟,韩姝,李艳华,谢小燕,施双双,陈琳,白慈贤,闫舫,南雪,王韫芳,裴雪涛.Wnt3a转基因基质细胞的建立及其对脐血CD34^+造血干/祖细胞扩增作用的研究[J].生物化学与生物物理进展,2005,32(9):835-841. 被引量：5
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第三军医大学学报
2008年 第17期

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