期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

黄芩素对染氟成骨细胞氧化应激水平的影响 被引量：2

Protective effects of baicalein on fluoride-induced oxidative stress in osteoblasts
下载PDF
导出
摘要 目的探讨黄芩素在染氟成骨细胞细胞内氧化还原水平的变化中发挥的抗氧化作用。方法观察染氟成骨细胞内脂质过氧化产物(MDA)及抗氧化酶(SOD、GPX)活性在黄芩素作用前后的变化。结果低浓度氟和高浓度氟均在成骨细胞引起不同程度的氧化应激态变化,联和应用黄芩素降低了染氟成骨细胞内的氧化应激水平。结论黄芩素很可能清除了成骨细胞内由氟诱导产生的过多氧自由基,进而减弱了不同浓度氟对成骨细胞的毒性作用。 Objective To study the effects of baicalein on oxidative stress in osteoblasts induced by fluoride. Methods To observe the changes of the contents of MDA and the activities of SOD, GPX in osteoblasts exposed to different concentration of fluoride with or without baicalein. Results The degree of oxidative stress induced by fluoride with baicalein was inferior to that induced by fluoride in osteoblasts. Conclusions The reactive oxygell species generated by fluoride in osteoblasts are probably scavenged by baicalein, thus baicalein probably reduced the toxie effects.
作者 张秀云 徐辉 李向军 张文博 李广生
机构地区 吉林大学地方病研究所
出处 《中国地方病防治》 北大核心 2008年第4期254-256,共3页 Chinese Journal of Control of Endemic Diseases
基金 国家自然科学基金资助(青年基金 项目号30700688)
关键词 氟化物 成骨细胞 氧化应激 黄芩素 Fluoride Osteoblasts Oxidative stress Baicalein
分类号 R599.9 [医药卫生—内科学]
  • 相关文献

参考文献15

  • 1Shivarajashankara YM, Shivarajashankara AR, Rao SH, et al. Oxidative stress in children with endemic skeletal fluorosis[J]. Fluoride,2001, 34(2):103-107.
  • 2Patel PD, Chinoy NJ. Influence of fluoride on biological free reactions in ovary of mice and its reversal [ J]. Fluoride, 1998, 31: S27.
  • 3Shama A, Chinoy NJ. Role of free radical in fluoride - induced toxicity in liver and kidney of mice and its reversal [J]. Fluoride,1998, 31: S26.
  • 4Rzeuski R, Chlubek D, Machoy Z. Interactions between fluoride and biological free radical reaction [ J]. Fluoride, 1998, 31 : 43-45.
  • 5高中洪,黄开勋,徐辉碧.黄芩黄酮对自由基的清除作用的ESR研究[J].华中理工大学学报,1999,27(1):97-99. 被引量：37
  • 6Yoshino M, Murakami K. Interaction of iron with polyphenolic compounds: application to antioxidant characterization [J]. AnalBiochem,1998, 257:40-44.
  • 7李彤,张秀云,徐辉,井玲.氟对体外培养成骨细胞氧化应激和增殖活性的影响[J].中国实验诊断学,2007,11(2):194-197. 被引量：6
  • 8Fareley JR, Wergedal JE, Baylink DJ. Fluoride directly stimulates proliferation and alkaline phosphatase activity of bone - forming cells [ J ]. Science, 1983,222 : 330-332.
  • 9Chavassieux P, Boivin G, Serre CM, et al. Fluoride increases rat osteoblast function and population after in vivo administration but not after in vitro exposure[ J]. Bone,1993,14: 721-725.
  • 10Nakamura H, Nakamura K, Yodoi J. Redox regulation of cellular activation [ J ]. Annu Rev Immunol, 1997, 15 : 351-369.

二级参考文献11

  • 1李广生,井玲,徐辉.地方性氟中毒发病机制的研究进展[J].中华病理学杂志,2005,34(10):632-634. 被引量：47
  • 2徐辉,井玲,张承巍,齐玲,李广生.染氟成骨细胞的双向电泳和质谱鉴定[J].中国地方病学杂志,2006,25(1):35-38. 被引量：16
  • 3徐辉 李兴华 李广生.氧化应激在慢性氟中毒发病机制中的作用[A].见:李广生主编.地方性氟中毒发病机制[M].北京:科学出版社,2004.174-181.
  • 4Cao Guohua，Free Radic Biol Med，1997年，22卷，5期，749页
  • 5Shahidi F，Crit Rev Food Sci Nutr，1992年，32卷，67页
  • 6于留荣，药物分析杂志，1983年，3卷，1期，18页
  • 7Kondo N,Nakamura H,Masutani H.et al.Redox regulation of human thioredoxin network[J].Antioxid Redox Signal,2006,8 (9-10):1881.
  • 8Tu BP,Weissman JS.Oxidative protein folding in eukaryotes:mechanisms and consequences[J].J Cell Bio 2004,164(3):341.
  • 9Nakamura H,Nakamura K,Yodoi J.Redox regulation of cellular activation[J].Annu Rev Immunol,1997,15:351.
  • 10Cumming RC,Andon NL,Haynes PA,et al.Protien disufide bond formation in the cytoplasm during oxidative stress[J].J Biol Chem 2004,279(21):21749.

共引文献41

同被引文献22

  • 1周波,王晓红,陈丽丽,郭连营,张卓,徐超.玉米紫色植株色素体外抗氧化活性实验研究[J].现代食品科技,2007,23(4):23-25. 被引量：26
  • 2Czekanska EM, Stoddart MJ, Richards RG, et al. In search of an osteoblast cell model for in vitro research [J]. European Cells and Materials, 2012, 24 (4): 1-17.
  • 3Jacob JK, Tiwari K, Correa-Betanzo J, et al. Biochemical basis for functional ingredient design from fruits [ J ]. Annu Rev Food SciTechnol, 2012, 3: 79-104.
  • 4Hwang YP, Choi JH, Yun H J, et al. Anthocyanins from purple sweet potato attenuate dimethylnitrosamine-induced liver injury in rats by inducing Nrf2-mediated antioxidant enzymes and reducing COX-2 and iNOS expression [ J]. Food Chem Toxicol, 2011, 49 (1): 93-99.
  • 5WAALKES M P, COOGAN T P, BARTER R A. Toxicological principles of metal carcinogenesis with special emphasis on cadmi- um[J]. Crit Rev Toxicol, 1992, 22 (3/4) : 175 -201.
  • 6KAMIYAMA T, MIYAKAWA H, LI J P, et al. Effects of one - year cadmium exposure on livers and kidneys and their relation to glutathione levels[J]. Res Commun Mol Pathol Pharmacol, 1995, 88 (2): 177-186.
  • 7SHAIKH Z A, VU T T, ZAMAN K. Oxidative stress as a mecha- nism of chronic cadmium - induced hepatotoxicity and renal toxicity and protection by antioxidants [ J ]. Toxicol Appl Pharmacolo, 1999, 154 (3) : 256 -263.
  • 8FOTAKIS G, TIMBRELL J A. Role of trace elements in cadmium chloride uptake in hepatoma cell lines [ J ]. Toxicol Lett, 2006, 164 (2): 97-103.
  • 9FUNAKOSHI T, UEDA K, SH[MAI3A H, et al. Effects of dithio- carbamates on toxicity of cadmium in rat primary hepatocyte cultures [J]. Toxicology, 1997, 116 (1/2/3):99-107.
  • 10HATCHER E L, CHEN Y, KANG Y J. Cadmium resistance in A549 cells correlates with elevated glutathione content but not an- tioxidant enzymatic activities[ J]. Free Radic Biol Med, 1995, 19 (6) : 805 -812.

引证文献2

二级引证文献6

中国地方病防治
2008年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部