小滨麦易位系山农0096抗条锈基因的微卫星标记和染色体定位

Microsatellite Marker and Chromosomal Location of the Yellow Rust Resistance Gene in Tritileymus Translocation Line Shannong0096

从普通小麦品种烟农15与八倍体小滨麦杂交后代中选育的小滨麦种质系山农0096,综合性状优良,高抗条锈病,经鉴定为导入了滨麦草染色体的小片段易位系。通过抗性接种鉴定和遗传分析,推测山农0096的条锈病抗性由显性单基因控制,抗条锈病基因来源于滨麦草,暂将其表示为YrSn0096。利用F2分离群体分组法(BSA)对山农0096中的抗条锈病基因进行了微卫星标记分析,从1261对SSR引物中筛选出引物BARC236-4A能在滨麦草、八倍体小滨麦和山农0096中扩增出特异性DNA片段Xbarc236-4A255bp;利用该标记检测山农0096×辉县红F2群体的单株DNA,根据扩增带型,利用软件MAPMAKER3.0确定标记Xbarc236-4A与滨麦草抗条锈基因的遗传距离为5.0cM,并将该抗条锈病基因定位在4A染色体上。

Shannong0096, a derivative of wheat- Octoploid Tritileymus, with useful agronomic characteristics, excellent yellow rust resistance, has been identified to be a translocation line with a small alien fragment ofL. mollis. The results of inoculation identification and genetics analysis showed that the yellow rust resistance of Shannong0096 was controlled by a single dominant gene derived from L. mollis which was temporarily designated YrSn0096. Using method of bulk segregant analysis, the Yr in Shannong0096 was identified, SSR tagged, and located. 1 261 microsatellite markers was screened, BARC236-4A was selected which has specific amplified products Xbarc236-4AE255bp in L. moUis, Octoploid Tritileymus and Shannong0096. The polymorphic marker was further used to analyze susceptible and resistant individuals of Shannong0096 ×Huixianhong F2 segregating population, and then software MAPMAKER3.0 established that the genetic distance between Xbarc236-4A and the target gene is 5.0 cM. The rust resistance gene was presumably placed on chromosome 4A.