利用MAS技术改良四个水稻恢复系及其抗病性分析

Improve and Resistance Analyses of Four Rice Restores by MAS

水稻白叶枯病作为世界范围内的水稻三大病害之一,给水稻的生产带来了严重的损失。培育抗病品种是解决水稻抗病性的主要措施。目前国内的杂交水稻携带的抗性基因普遍单一,长期大规模推广的情况下容易造成抗性的丧失从而引起某种病害的大爆发。因此,拓宽抗谱,增强抗性并且抗性持久是今后水稻育种的主要方向之一。实验表明,将不同来源的抗性基因通过杂交或者转移聚合到同一单株中能够明显的增强抗性并且拓宽抗谱,这对培育广谱高抗的水稻来说无疑是一个很好的途径。本研究利用IRBB60(Xa-21和Xa-4)和WBB1(Xa-23)作为抗性基因供体,以宜恢1577、宜恢3551、盐恢559和明恢63等4个常用的水稻恢复系作为轮回亲本,通过3次回交和2次自交,在各个后代中分别用与三个目标基因Xa-23、Xa-21、Xa-4紧密连锁/共分离的标记RM206、pTA248、MP12在各个后代群体中进行选择并辅以接种验证,得到了抗性大幅度提高的改良后代。获得的改良后的宜恢1577、宜恢3551、盐恢559、明恢63双基因纯合累加系的抗病能力比不含任何抗性基因的对照株系大大加强,接种致病菌系P1后的病斑长度从分别从14.9cm、12.8cm、13.5cm、14.1cm下降到1.49cm、0.92cm、1.54cm、1.38cm;接种致病菌系P6后的病斑分别从14.8cm、13.2cm、13.6cm、14.8cm下降到1.47cm、1.12cm、1.33cm、1.71cm。并且对C1￣C7菌系的抗性也有了极大的提高。充分显示了多种抗性基因聚合后产生的强大抗性,也为以后生产中提供抗性优良的亲本提供了可能。

The rice bacterial blight, as one of main rice disease it made great lose in rice produce in the world. Breeding new species rice with high resistance is an efficient method to solve the problem. Domestic hydride rice inherent only few of resistance genes, it can indicate some disease influence in the condition of publicize long term and large scale. So widen resistance spectrum, enhance and sustain resistance is the develop trends of rice breeding. The experiment show that pyramiding several different resistance genes into one line can gain above aim. So it is an efficient way to breed new species in rice breeding. In this research we reconstructed 6 rice restores lines by MAS while IRBB6 and WBB1 as gene provider and the 6 restorers as backcross parent, after selected by markers RM206, p TA 248 and MP12 the resistance of BB was improved greatly. Among the four improved restores, Yihui1577, Yihui3551, Yanhui559 and Minghui63, the average lesion length declined from 14.9 cm, 12.8 cm, 13.5 cm, 14.1 cm to 1.49 cm, 0.92 cm, 1.54 cm, 1.38 cm by Inoculating P1. And also average lesion length declined from 14.8 cm, 13.2 cm, 13.6 cm, 14.8 cm to 1.47 cm, 1.12 cm, 1.33 cm, 1.71 cm by Inoculating P6. It showed that pyramiding muti-gene into same line can create new species with high resistance, it Can provide a good pertains in the hydride rice produce.