唐菖蒲体细胞胚的诱导及植株再生

Somatic Embryogeenesis and Plantlet Regeneration in Gladious

以唐菖蒲球茎芽切片为外植体,经体细胞胚发生途径,进行胚性愈伤组织诱导、胚状体的诱导、胚状体发育过程及植株再生的研究。胚状体诱导培养基为MS+2,4-D1.0mg/L+TDZ0.2mg/L,诱导率为68.3%;将产生的胚状体首先接种于MS培养基使其充分发育,之后转入MS+6-BA2.0mg/L培养基中诱导发芽,在转入MS培养基中使其形成完整植株。采用石蜡切片法和临时压片法对胚状体的发育过程进行了观察发现,首先外植体表层薄壁细胞经脱分化恢复分生能力,形成愈伤组织,随后在愈伤组织表面形成许多瘤状突起即胚性细胞团,胚性细胞团继续发育成球形胚、盾形胚,最后发育成熟形成完整植株。

Taking the slice of Gladious corms as explants, the regenerated way of plant was selected somatic embryogenesis. MS＋2,4-D 1.0 mg/L＋TDZ 0.2 mg/L was the best culture medium that the induction of somatic embryo, the rate was 68.3%. After that there was three steps to complete the culture： First, the regenerated somatic embryo were cultured in the MS medium to growth enough; Second, the growful somatic embryo was cultured in the MS＋6-BA 2.0 mg/L to induce buds; Third, the regenerated plants were obtained in the MS medium. The appearance and growth of somatic embryo were observed through paraffin slices and press slices. Histological observations showed that parenchyma cells first experienced dedifferentiation and followed with callus-forming, ensued with embryogenic cell masses which like a tubercular structure, and these embryogenic cell masses experienced globular, scutellate stages and developed into whole plantlets.