水稻矮秆突变体sde(t)的遗传分析与基因初步定位

Genetic Analysis and Molecular Mapping of sde(t) in Rice (Oryza sativa L.)

矮生突变体的引入掀起了第一次"绿色革命"。但近年来,在水稻育种中矮生基因遗传单一的问题越来越突出,已经严重影响了水稻产量的持续提高。从籼稻品种中籼3037中发现一个矮秆自然突变体,该矮秆突变体和中花11杂交F2的遗传分析表明该材料的矮秆性状由1对隐性单基因控制,并暂命名为sde(t)。利用已有的INDEL分子标记将sde(t)基因定位在水稻1号染色体的长臂上,然后通过扩大群体和新发展的INDEL与CAPS标记,将sde(t)基因定位在2个INDEL标记之间,两者间的物理距离大约是400kb。

The introduction of semidwarf rice led to the first ‘green revolution’, but few new dwarf gene was introduced into rice breeding until recently. A natural rice dwarf mutant, derived from an indica cultivar Zhongxian3037, was crossed with a japonica cultivar Zhonghua11 to investigate the genetic mode and map the gene. Genetic analysis indicated that the phenotype of the dwarf mutant was controlled by a pair of recessive gene and designated tentatively as sde（t）. Polymorphic analysis of INDEL markers demonstrated that the sde（t） gene was on the long arm of chromosome 1. With a larger mapping population, more CAPS and INDEL markers, the sde（t） gene was further mapped between two INDEL markers with a physical region of about 400 kb.