香蕉愈伤组织原生质体分离研究

Study on Embryogenic Callus Protoplast Isolation of Banana

本实验以巴西香蕉和皇帝蕉胚性愈伤组织为材料,对影响其原生质体产率和活力的因素进行了研究。结果表明:对诱导巴西蕉及皇帝蕉胚性愈伤进行原生质体分离,分别用CPW+13%甘露醇+1.6%纤维素酶+0.9%果胶酶酶解液处理巴西蕉愈伤组织12h,用CPW+11%甘露醇+1.8%纤维素酶+1.0%果胶酶酶解液处理皇帝蕉愈伤组织12h。巴西蕉产率最高达2.56×107个/mL,活力达61.5%;皇帝蕉产率达2.64×107个/mL,活力达63.4%。

Protoplasts of Musa AAA group cv. Brazilian and Musa paradisiacal AA were isolated from embryogenic calli. The different factors affecting yield and viability of the protoplasts were investigated. The results showed that the protoplasts with higher yield and viability were obtained by treating the Musa AAA group cv. Brazilian and Musa paradisiacal AA embryogenic calli with a mixture of celluase onozuka and macerozyme onozuka. The Musa AAA group cv. Brazilian callus with CPW＋13% mannitol＋1.6% celluase onozuka＋0.9% macerozyme onozuka for 12 h, Musa paradisiacal AA callus with CPW＋11% mannitol＋1.8% celluase onozuka＋1.0 macerozyme onozuka for 12 h, respectively. The best protoplasts yield of Musa AAA group cv. Brazilian was 2.56×10^7/mL and the viability was 61.5%, and the protoplast yield of Musa paradisiacal AA was 2.64×10^7/mL and the viability was 63.4%.