胃癌前病变患者根除幽门螺杆菌后Cx32和Cx43的表达

Expressions of connexin 32 and connexin 43 in patients with gastric precancerous lesion after eradication of Helicobacter pylori

目的:观察胃癌前病变(PL)患者幽门螺杆菌(H.pylori)根除前后Cx32,Cx43表达变化。方法:采用免疫组织化学SABC法检测33例慢性浅表性胃炎(CSG)、88例胃癌前病变(PL)患者H.pylo-ri根除前后胃黏膜活检标本Cx32,Cx43表达水平。结果:PL组Cx32,Cx43阳性表达率(分别为51.1%和54.5%)和阳性表达强度均低于CSG组(分别为100%和93.9%,P<0.05)。胃癌前病变H.pylori阳性组Cx32,Cx43阳性表达率(分别为41.4%和44.8%)和阳性表达强度均低于H.pylori阴性组(分别为70%和73.3%,P<0.05)。胃癌前病变组H.pylori根除者Cx32,Cx43阳性表达率(分别为97.9%和91.7%)较治疗前升高(分别为41.4%和44.8%,P<0.05);未根除者治疗后(分别为40%和50%)与治疗前比较差异无统计学意义(P>0.05)。结论:胃癌前病变患者Cx32,Cx43表达降低,H.pylori根除可上调Cx32,Cx43表达。

Objective To observe the change in expressions of connexin 32 and connexin 43 after the eradication of Helicobacter pylori （H. pylori ） in patients with gastric precancerous lesion. Methods The expressions of connexin 32 and connexin 43 in gastric mucosa specimens biopsy under endoscopy were detected by immunohistochemistry. The expressions of connexin 32 and connexin 43 were detected before and after the eradication of H. pylori in 88 patients with gastric precancerous lesion, and in 33 patients with chronic superficial gastritis （ CSG ）. Results The positive expression rates and the expressional intensity of connexin 32 and connexin 43 in patients with gastric precancerous lesions （51. 1% and 54. 5%） were lower than those in patients with CSG （100% and 93.9% , P 〈 0.05 ）. In patients with gastric precancerous lesions, the positive expression rates and the expressional intensity of connexin 32 and connexin 43 in H. pylori positive group （41.4% and 44.8% ） were lower than those in H. pylori negative group （70% and 73.3%, P 〈 0.05 ）. In gastric precancerous lesions group, the positive expression rates of connexin 32 and connexin 43 in H. pylori positive group before the eradication therapy （41.4% and 44. 8 % , respectively ） was lower than those after the eradication of H. pylori （97.9% and 91.7% ,P 〈0.05 ） ; in the eradi-cation failure group, the positive expression rates of connexin 32 and connexin 43 were 40% and 50%. The eradication failure group before the treatment and after the treatment had no statistical significance （P 〉 0. 05 ）. Conclusion The expressions of connexin 32 and connexin 43 in patients with gastric precancerous lesions are low, and the eradication of H. pylori can upregulate their expressions.