摘要
目的:探讨骨形成蛋白-2(bone morphogenetic protein-2,BMP-2)对人肺癌A549细胞膜型基质金属蛋白酶-1(membrane type 1-matrix metalloproteinase,MT1-MMP)表达的影响。方法:Western杂交检测MT1-MMP蛋白质表达。采用酶联免疫吸附法(enzyme-linked immunosorbentassay,ELISA)检测细胞培养液中活性基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)的浓度。结果:BMP-2可促进A549细胞MT1-MMP蛋白质表达,并呈剂量和时间依赖关系。ELISA进一步显示BMP-2可增加A549细胞培养液中活性型MMP-2的含量。结论:BMP-2可促进A549细胞MT1-MMP的表达及MMP-2的激活。肺癌组织中BMP-2表达的增加可通过促进肺癌细胞MT1-MMP表达及MMP-2激活而促进肺癌细胞的侵袭转移,此可能为肺癌侵袭转移的另一重要机制。
Objective To explore the effect of bone morphogenetic protein-2 (BMP-2) on the expression of membrane Type 1-matrix metalloproteinase (MT1-MMP) and the activation of matrix metalloproteinase-2 (MMP-2) in human A549 lung carcinoma ceils. Methods Western blot was used to analyse the expression of MT1-MMP protein levels in A549 ceils. Enzyme-linked immunosorbentassay was used to show the actions of BMP-2 on the activation of MMP-2 in A549 ceils. Results Treatment with BMP-2 in A549 ceils caused a dose- and time-dependent increase in the expression of MT1-MMP protein. BMP-2 dose-dependently activated MMP-2 in A549 ceils. Conclusion BMP-2 might promote MT1-MMP expression and MMP-2 activation in lung carcinoma ceils, which can lead to the increase of extraeellular matrix followed by acceleration of tumor cell migration and invasion. It is probably another key mechanism of BMP-2 upregulated lung carcinoma cell migration.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2008年第7期634-637,共4页
Journal of Central South University :Medical Science
基金
中国博士后科学基金(20070410995)~~