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骨形成蛋白-2对肺癌A549细胞膜型基质金属蛋白酶-1表达的影响 被引量：1

Effect of bone morphogenetic protein-2 on the expression of membrane Type 1-matrix metalloproteinase in human A549 lung carcinoma cells
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摘要 目的:探讨骨形成蛋白-2(bone morphogenetic protein-2,BMP-2)对人肺癌A549细胞膜型基质金属蛋白酶-1(membrane type 1-matrix metalloproteinase,MT1-MMP)表达的影响。方法:Western杂交检测MT1-MMP蛋白质表达。采用酶联免疫吸附法(enzyme-linked immunosorbentassay,ELISA)检测细胞培养液中活性基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)的浓度。结果:BMP-2可促进A549细胞MT1-MMP蛋白质表达,并呈剂量和时间依赖关系。ELISA进一步显示BMP-2可增加A549细胞培养液中活性型MMP-2的含量。结论:BMP-2可促进A549细胞MT1-MMP的表达及MMP-2的激活。肺癌组织中BMP-2表达的增加可通过促进肺癌细胞MT1-MMP表达及MMP-2激活而促进肺癌细胞的侵袭转移,此可能为肺癌侵袭转移的另一重要机制。 Objective To explore the effect of bone morphogenetic protein-2 （BMP-2） on the expression of membrane Type 1-matrix metalloproteinase （MT1-MMP） and the activation of matrix metalloproteinase-2 （MMP-2） in human A549 lung carcinoma ceils. Methods Western blot was used to analyse the expression of MT1-MMP protein levels in A549 ceils. Enzyme-linked immunosorbentassay was used to show the actions of BMP-2 on the activation of MMP-2 in A549 ceils. Results Treatment with BMP-2 in A549 ceils caused a dose- and time-dependent increase in the expression of MT1-MMP protein. BMP-2 dose-dependently activated MMP-2 in A549 ceils. Conclusion BMP-2 might promote MT1-MMP expression and MMP-2 activation in lung carcinoma ceils, which can lead to the increase of extraeellular matrix followed by acceleration of tumor cell migration and invasion. It is probably another key mechanism of BMP-2 upregulated lung carcinoma cell migration.
作者 唐四元
机构地区 中南大学护理学院
出处 《中南大学学报（医学版）》 CAS CSCD 北大核心 2008年第7期634-637,共4页 Journal of Central South University ：Medical Science
基金 中国博士后科学基金(20070410995)~~
关键词 骨形成蛋白-2 膜型基质金属蛋白酶-1 人肺癌A549细胞 bone morphogenetic protein-2 membrane type 1-matrix metalloproteinase A549 lung carcinoma cells
分类号 R734.2 [医药卫生—肿瘤]
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参考文献14

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同被引文献26

  • 1田东波,胡国洪,肖伟民,曾峰,潘桂常,夏明凯.MMP 2、MMP 9在非小细胞肺癌中的表达及其与临床病理特征的关系[J].现代临床医学生物工程学杂志,2005,11(3):170-173. 被引量：3
  • 2Kiesslich T, Pichler M, Neureiter D. Epigenetic control of epithelial-mesenchymal-transition in human cancer[J]. Mol Clin Oncol, 2013, 1 (1):3-11.
  • 3Orlichenko LS, Radisky DC. Matrix metalloproteinases stimulate epithelial- mesenchymal transition during tumor development[J]. Clin Exp Metastasis, 2008, 25(6):593-600.
  • 4Zheng Y, Wang X, Wang H, et al. Bone morphogenetic protein 2 inhibits hepatocellular carcinoma growth and migration through downregulation of the PI3K/AKT pathway[J]. Tumour Biol, 2014, 35(6): 189-198.
  • 5Jin H, Pi J, Huang X, et al. BMP2 promotes migration and via cytoskeleta nvasion of breast cancer cells reorganization and adhesion decrease: an AFM investigation[J]. Appl Microbiol Biotechnol, 2012, 93(4):1 715-1 723.
  • 6Kang MH, Kim JS, Seo JE, et al. BMP2 accelerates the motility and invasiveness of gastric cancer cells via activation of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway[J]. Exp Cell Res, 2010, 316(1):24-37.
  • 7Chen X, Liao J, Lu Y, et al. Activation of the PI3K/Akt pathway mediates bone morphogenetic protein 2-induced invasion of pancreatic cancer cells Panc-1 [J]. Pathol Oncol Res, 2011, 17(2):257-261.
  • 8Langenfeld EM, Bojnowski J, Perone J, et al. Expression of bone morphogenetic proteins in human lung carcinomas[J]. Ann Thorac Surg, 2005, 80(3):1028-1032.
  • 9Fei ZH, Yao CY, Yang XL, et al. Serum BMP-2 up- regulation as an indicator of poor survival in advanced non-small cell lung cancer patients[J]. Asian PacJ Cancer Prev, 2013, 14(9):5293-5299.
  • 10Warrington SJ, Strutt H, Strutt D. The frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2[J]. Development, 2013, 140(5):1045-1054.

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中南大学学报（医学版）
2008年 第7期

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