吕泗渔场三疣梭子蟹自然群体同工酶与ISSR遗传多样性分析

Analysis on genetic diversity of Portunus triuberbuculatus population from Lvsi fishing region

采用不连续垂直板聚丙烯酰胺凝胶电泳方法和ISSR分子标记技术,检测分析了南黄海吕泗渔场海域三疣梭子蟹自然群体遗传多样性状况。结果表明:(1)三疣梭子蟹EST同工酶在肌肉和肝脏的表达具有明显的组织特异性。5种同工酶(LDH、GDH、EST、MDH、ME)在肌肉及肝脏两种组织中共检测到12个基因座位,其中5个基因座位Est-1、Est-2、Mdh-2、Gdh-2、M e-3为多态。(2)用6个ISSR随机引物的PCR扩增分析得出:平均每个位点的有效等位基因数Ne=1.548,Ne i’s基因多样性指数H=0.316,Shannon多样性信息指数Hsp=0.468。两种分析结果一致表明该三疣梭子蟹自然群体杂合度较高,遗传多样性较为丰富。

Vertical polyacrylamide gel electrophoresis （ PAGE ） and inter-simple sequence repeat （ISSR） were used to investigate the genetic diversity of Portunus triuberbuculatus collected from Lüsi fishing region in the southern Yellow Sea. Results indicated that （1）Significant tissue specificity was observed in EST isozyme expression in muscle and liver; among 5 isozymes （ LDH, GDH, EST, MDH, ME） of muscle and liver, twelve loci were detected, of which five（Est-1, Est-2, Mdh-2, Gdh-2, Me-3） were found polymorphic. （2） 45 bands were amplified by 6 informative and reliable primers, 37 of which were polymorphic, the mean effective allele number（Ae） was 1. 548, Nei＇ s gene diversity（H） was 0. 316, Shannon＇ s diversity index （Hsp） was 0.468. Results of both methods demonstrated a rich genetic diversity in this wild stock .