肌肉注射维生素C对三角帆蚌血淋巴抗氧化酶活力的影响

The effect of Vitamin C injection on the activities of antioxidant enzymes in the hemolymph of Hypriopsis cumingii

在室内水族箱养殖条件下,研究了肌肉注射不同剂量维生素C(Vc)对三角帆蚌血淋巴中3种主要抗氧化酶活力随时间的变化。结果表明:Vc对三角帆蚌血淋巴过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)3种酶的活性均有显著影响(P<0.05)。当Vc注射剂量分别为15μg/g、30μg/g和45μg/g时,在注射后的12 h、24 h和48 h时间点,SOD酶的活性先升后降,GSH-PX酶的活性随Vc注射剂量的加大而降低,总体呈逐渐下降趋势,而CAT酶的活性随Vc注射剂量的加大而增强。

Studies were carried out on the effect of Vc on the activities of three major antioxidant enzymes in the hemolymph of Hypriopsis cumingii on the condition of indoor aquarium culture. The results showed that： Vc had a notable impact on the activities of Catalase （CAT）, Superoxide dismutase （SOD） and Glutathione peroxidase （GSH-PX） enzymes （P 〈 0.05 ）. Under the conditons of the injected doses of Vc 15 ug/g, 30 ug/g and 45 ug/g, at 12 h, 24 h and 48 h after injecting, the activity of SOD enzyme would rise and then dropped, the activity of GSH-PX enzyme dropped down with the rise of injected dose of Vc, but always came down as a whole. However, the activity of CAT enzyme increased with the rise of injected dose of Vc.