牛磺酸对鲤非特异性免疫及抗氧化能力的影响

Effect of taurine on the non-specific immunity and antioxidative competence of carp

采用单因子浓度梯度法,将牛磺酸以0.05%、0.10%、0.15%、0.20%的剂量添加到基础饲料中,以基础饲料为对照,饲喂鲤(体重40.73±3.92 g)30 d后,测定血清和肝胰脏的溶菌酶活力、超氧化物歧化酶活力、过氧化氢酶活力和丙二醛的含量。结果表明:牛磺酸可以提高鲤血清和肝胰脏的溶菌酶活力、超氧化物歧化酶活力、过氧化氢酶活力,降低鲤血清和肝胰脏中的丙二醛含量,其中0.10%添加组效果最好,极显著地提高鲤血清和肝胰脏的溶菌酶活力(P<0.01),比对照组分别提高9.74%、8.04%;极显著地提高鲤血清和肝胰脏的超氧化物歧化酶活力,比对照组分别提高18.00%、14.40%;极显著地提高鲤血清的过氧化氢酶活力,显著地提高鲤肝胰脏的过氧化氢酶活力(P<0.05),比对照组分别提高16.87%、9.98%;极显著地降低鲤血清和肝胰脏的丙二醛含量,比对照组分别降低25.28%、15.21%。饲料中适宜剂量牛磺酸的添加,能显著增强鲤的抗氧化能力和非特异性免疫功能,减轻鱼体的脂质过氧化作用。

Carp （ body weight 40.73 ± 3.92 g） were fed on formulated diet added with taurine at different levels of 0.05% , 0. 10% , 0. 15% , 0.20% respectively. The lysozyme activities, superoxide dismutase （SOD） activities, catalase （CAT） activities and malondialdehyde （MDA） content of carp＇s serum and hepatopancreas were determined after 30 days. The result showed that taurine very significantly increased the lysozyme, SOD, CAT activities of carp＇s serum and hepatopancreas（P 〈0.01 ） , very significantly decreased the MDA content of carp＇ s serum and hepatopancreas（P 〈 0.01 ）. The lysozyme activities of carp＇ s serum and hepatopancreas of the treated groups -0. 10% were higher than those of the control 9.74%, 8.04% respectively. The SOD activities of carp＇ s serum and hepatopancreas of the treated groups -0.10% were higher than those of the control 18.00% , 14.40% respectively. The CAT activities of carp＇ s serum and hepatopancreas of the treated groups - 0. 10% were higher than those of the control 16. 87% , 9. 98% respectively. The MDA content of carp＇ s serum and hepatopancreas of the treated groups -0.10% were lower than those of the control 25.28% , 15.21% respectively. The proper taurine can increase the antioxidative competence and non-specific immunity of carp, and decrease lipid peroxidation of carp.