CFD-ACE+软件对PCR微芯片传热过程的数值模拟和分析

Simulation and Analysis of Heat Transfer for PCR Microchip by Using CFD-ACE+ Software

针对基于MEMS技术的PCR微芯片温度控制难的问题,通过对该芯片的传热过程进行简化并建立物理模型和数学模型,对芯片内部的热传导和温度变化过程进行了理论推导分析。利用CFD-ACE+软件对芯片的热传导过程进行了数值模拟。模拟结果表明,该芯片具有较高的升、降温速度。芯片在自行构建的温度控制系统下进行了热循环反应,对GUS基因成功实现了扩增。

A novel PCR （polymerase chain reaction） microchip was designed and fabricated by MEMS technology, which was integrated with micro heaters and temperature sensors in chip for rapid thermal cycling. Heating and cooling rates are evaluated theoretically by setting up a simplified heat transfer model of the microchip. The processes of heat conduction and diffusion are simulated by using CFD-ACE ＋ software. Simulation shows that the chip has high temperature rising and cooling rates. By combining peripheral thermal control system, the GUS gene is successfully amplified in chip.