二苯乙烯荧光蓝-S荧光猝灭测定微量锌(Ⅱ)

Fluorescence Quenching of Stilbenefluoblue s for the Determination of Trace Zinc(Ⅱ)

研究了二苯乙烯荧光蓝－Ｓ荧光猝灭测定微量锌（Ⅱ）的实验条件．在ｐＨ６．０的Ｈ３ＰＯ４－Ｈ３ＢＯ３－ＨＡｃ－ＮａＯＨ缓冲溶液中，试剂的最大激发波长λｅｘ＝３６７ｎｍ，最大荧光发射波长λｅｍ＝４５７ｎｍ；Ｚｎ（Ⅱ）与试剂生成的１∶２的配合物使试剂的荧光强度减弱；利用荧光猝灭法测定微量锌（Ⅱ），线性范围为０～０．８μｇ／１０ｍｌ；方法用于测定食品中、人发中的微量锌（Ⅱ），结果满意．

The condition of the fluorescence quenching of Zn(Ⅱ) stilbenefluoblue s(2,2′ Disulfo 4,4′ diaminostilbene N,N,N′,N′ tetraacetic acid disodium salt,C 22 H 20 N 2O 14 S 2Na 2) system for determining trace amounts of Zn(Ⅱ) have been studied.The maximum excitation wavelength of reagent is 367 nm and its maximum emission wavelength is 457 nm in the H 3PO 4 H 3BO 3 HAc NaOH buffer solution at pH 6.0.The formation of Zn(Ⅱ) stilbenefluoblue s complex which lead to the decrease of fluorescence indensity of reagent.The liner relationship is obeyed in the range of 0￣0.8 μg/10 ml for Zn(Ⅱ).This method has been applied to be determination of trace Zn(Ⅱ) in degrease milk powder,human hair and nutritive table salt of added Zn(Ⅱ) and the relative standard deviation is 3.4%,2.8% and 1.2%,respectively.