猪细小病毒PCR检测方法的建立及应用

Development of PCR Technique for Detecting Porcine Parvovirus Infection

根据已报道猪细小病毒基因组序列,设计合成了一对特异引物,通过对影响PCR扩增因素的筛选,确定PCR检测的最佳条件,成功扩增出预期的1980bp片断。特异性和敏感性试验结果发现:该方法特异、敏感,可以检测到0.9TCID50、0.001个HA的病毒。用该方法对用ZH株免疫妊娠母猪后第7、14、21天血液及胎儿样品进行检测,在血液和所采组织样品中均未检测到PPV病毒抗原,说明ZH株免疫后不产生病毒血症,也不能通过胎盘垂直传染给仔猪。

A pair of primers was derived from the published DNA sequences. The PCR method was developed for the detection of PPV DNA. The results revealed that the expected 1980 bp fragment could be amplified from passage ceils. The experiment result indicate that PCR is qualified with high specificity and sensitiveness, 0.9 TCID50 virus or 0.001 HA virus can be tested. Using PCR to test blood and fetuses of immuned gilts and sow with strain ZH, no virus antigen can be found in blood and issues, suggesting that there is no viremia and vertical transplaeental infection.