植物病毒抗血清的制备及IgG纯化与酶标技术研究

A STUDY ON THE TECHNIQUES OF THE PREPARATION OF PLANT VIRUS ANTISERUM AND THE PURIFICATION OF IgG

以烟草花叶病毒（TMv）为例研究了制备植物病毒抗血清的一般方法．以家兔和小白鼠为免疫动物，运用饱和硫酸接沉淀法制备出高效价的兔和瓦抗TMV血清，并运用先进的ELISA方法测定了抗血清中抗体IgG的效价；用醋酸纤维凝胶电泳技术分析了抗血清中抗体的lgG纯度，应用免疫双扩散法检测了提纯的IgG的生物活性．同时还运用成二醛一步法制出高结合率的IgG与过氧化氢酶（HRP）标记的抗体，形成了一整套制备抗血清、纯化IgG并对IgG进行酶标的技术，为植物病毒检测提供了快速简便的方法．

We studed the techniques of the preparation of plant virus antiserum and the pu-rification of IgG. Useing rabbit and mouse as immune animals,TMV as a antigene,we pre-pared anti-TMV serum. Assayed this antiserum by enzyme linked immunosorbent assay(ELISA),we fund it had a high titre. From rabbit antiserm puri ficated the lgG with satura-tion (NH'),SO, solution preipitate method,and this pure IgG had a high purity and a high bi-ological vigour. Useing this pure IgG and horseradish peroxidase (HRP) we have had a en-zyme labeling antibody, and, such this antibody can widespreadly used in immunoenzyatictechniques about assays of plant virus.