摘要
目的测定蒲公英中绿原酸与咖啡酸的含量。方法采用Diamonsil C18色谱柱,流动相为乙腈-磷酸二氢钠缓冲液(13∶87,v/v),检测波长323 nm。结果绿原酸在2.4-48.0μg/mL(r=0.999 5),咖啡酸在1.0-20.0μg/mL(r=0.999 7)线性关系良好,咖啡酸、绿原酸的平均回收率分别为99.7%(RSD=0.8%)和100.8%(RSD=1.1%)。结论该方法准确、可靠、重现性好,可作为控制蒲公英药材质量的方法。
Objective RP-HPLC method was established to determine chlorogenic acid and caffeic acid in Herba taraxaci.Methods Diamonsil C18 column was used with a mixture of acetonitrile and sodium phosphate buffer(13∶87,v/v) as mobile phase.Results The linear ranges were 2.4~48.0 μg/mL(r=0.999 5),1.0~20.0 μg/mL(r=0.999 7) respectively.The recoveries were 99.7%(RSD=0.8%),100.8%(RSD=1.1%) respectively.Conclusion The assay method is simple,rapid and with good reproducibility and,it could provide the quality assessment for Herba taraxaci.
出处
《实用药物与临床》
CAS
2008年第4期259-260,共2页
Practical Pharmacy and Clinical Remedies