岷山红三叶愈伤组织诱导和植株再生研究

Studies on Callus Induction and Plant Regeneration of Minshan Red Clover

通过对岷山红三叶下胚轴和子叶再生体系建立的研究，获得愈伤组织诱导的最佳培养基为：Ms＋2mg／L 2，4D＋0．5mg／L 6-BA＋2％蔗糖＋0．6％琼脂，胚性愈伤组织诱导培养基为：MS＋0．1mg／L IAA＋0．5mg／L 6-BA＋2％蔗糖＋0．6％琼脂，芽诱导培养基为：B5＋0．06mg／L NAA＋2％蔗糖＋0．6％琼脂，每瓶可以长出2～10条茎，将茎转入生根培养基后愈伤继代可持续出芽。最佳的生根培养基为：1／2MS＋0．05mg／L NAA＋1．5％蔗糖＋0．8％琼脂。建立再生植株约需160d。

Tissue culture technique is the basis for plant breeding and genetic biodiversity research. Through the study in plant regeneration of hypocotyl and cotyledon of Trifolium pratense L. cv. Minshan, we found that the optimal callus induction medium was MS＋2 mg/L 2,4 D＋0. 5 mg/L 6-BA＋2% sucrose＋0.6% agar; the best medium for embryogenic callus induction was MS＋0.1 mg/L IAA＋0.5 rag/ L 6-BA＋2% sucrose ＋0. 6% agar; bud induction medium was BS＋0. 06 mg/L NAA＋2% sucrose＋ 0.6%. agar. In each bottle, 2 to 10 stems were obtained and then transferred into root induction medium, buds could continually grow. The optimal root induction medium was 1/2 MS＋0.05 mg/L NAA＋1.5% sucrose ＋0.8% agar. 160 d was needed for the establishment of plantlet.