COX-2和Survivin在食管癌组织芯片的表达及临床意义

Expression and clinical significance of COX-2 and survivin in esophageal carcinoma tissue micro-array

目的:检测食管癌组织中COX-2和Survivin蛋白的表达情况及其与食管癌患者临床病理特征的关系。方法:采用免疫组化EnVision法和组织芯片技术,检测手术切除的56例食管癌患者肿瘤组织中COX-2和Survivin蛋白的表达状况,分析其与临床病理特征之间的关系及COX-2和Survivin表达的相关性。结果:COX-2在食管癌组织表达主要分布于细胞质,少数位于细胞膜,而Survivin则分布于细胞核。COX-2和Survivin在食管癌组织阳性表达率分别为69.6%和71.4%,而在食管癌旁组织则无表达。COX-2表达与性别(P=0.543)、年龄(P=0.561)和肿瘤分化程度(P=0.216)无关,但与肿瘤浸润深度(P=0.046)、淋巴结转移(P=0.001)及TNM分期(P=0.001)密切相关。Survivin表达与性别(P=1.000)、年龄(P=1.000)和肿瘤分化程度(P=0.333)亦无关,与肿瘤浸润深度(P=0.020)、淋巴结转移(P=0.003)及TNM分期(P=0.002)具有相关性。COX-2和Survivin均为阳性表达35例(62.5%),呈正相关(P=0.000,r=0.614)。结论:COX-2和Survivin在食管癌中呈过度表达,并与肿瘤浸润深度、淋巴结转移和临床分期密切相关,两者表达呈正相关,提示COX-2和Survivin在肿瘤进展中可能有协同作用。

Objective：To study the expression of COX-2 and survivin in esophageal squamous cell carcinoma（ESCC）, and to analyze the relationships between protein expression and clinicopathologic factors. Methods：Fifty-six cases of ESCC sections were immunohistochemically stained using COX-2 and survivin antibodies with Envision non-biotin detection system and tissue micro-array （TMA）. Expression of COX-2 and survivin protein in ESCC, the relationship between two proteins expression, and the relationship between protein expression and clinicopathologic factors, were analyzed using SPSS 12.0 for windows software. Results： COX-2 was mainly expressed in the cytoplasm, occasionally found in the membrane. Survivin was expressed in the nucleus. Both COX-2 and survivin were not found in normal esophageal tissues surrounding tumor. The positive expression rate of COX-2 and survivin protein was 69. 6% （39/56） and 71.4% （40/56）, respectively. COX-2 expression was correlated with tumor infiltration depth （P = 0. 046）, lymph node metastasis（P = 0. 001 ） and TNM staging （ P = 0. 001 ）, but not correlated with sex （ P = 0.543 ）, age （ P = 0. 561 ） and tumor cell differential degree（P = 0. 216）. Survivin expression was correlated with tumor infiltration depth （P = 0. 020）, lymph node metastasis （ P = 0. 003 ） and TNM staging （ P = 0. 002 ）, but not correlated with sex （ P = 1. 000 ）, age （ P = 1. 000 ） and tumor cell differential degree（P = 0. 333）. Both COX-2 and survivin positive expression were found in 35 specimens of all 56 ESCCs （62. 5% ）. COX-2 expression was positively correlated with survivin expression （P = 0. 002, r = 0. 614 ）. Conclusion： This study showed that COX-2 and survivin expression were over-expressed in esophageal carcinoma, and correlated to tumor infiltration depth, lymph node metastasis and clinical staging. COX-2 expression was positively correlated with survivin expression, which indicated that these two proteins might play collaborative roles during ESCC progression.