摘要
目的:研究角蛋白19(K19)mRNA在直肠癌区域淋巴结微转移中的表达及其临床意义。方法:通过病理组织学及逆转录聚合酶链反应(RT-PCR)扩增K19mRNA对19例直肠癌患者的癌组织标本和51个淋巴结进行检测。结果:19例直肠癌患者癌组织均有K19mRNA表达;51个淋巴结中组织学阳性16个(31.4%),而RT-PCR阳性21个(41.2%),RT-PCR扩增K19mRNA及内参照β-actin后,所有癌组织及转移淋巴结显示221bp和461bp的扩增片断,而非肿瘤患者的8个淋巴结仅显示221bp扩增片断。结论:K19可作为检测直肠癌患者淋巴结微转移的分子标志物,RT-PCR扩增K19mRNA检测直肠癌区域淋巴结微转移可能是一种敏感、特异的检测方法,是对常规病理组织学方法的补充。
Objective：To evaluate the significance of K19 mRNA expression in diagnosing micrometastasis of regional lymph nodes in patients with rectal cancer. Methods：Tumor tissues of 19 cases of rectal cancer and 51 lymph nodes were studied by both histopathological examination and K19 mRNA RT-PCR. Results： K19 mRNA expressed in all speciens of 19 patients with rectal cancer, 16 examined lymphm nodes showed positive lymph node metastasis by routine histopathological examination （31.4%） ,but the number of positive lymph nodes detected by RT-PCR increased to 21 （41.2%）. After K19 and β-actin were amplificated by RT-PCR,the amplification fragments of 221bp and 461bp appeared in all cancer and metastatic lymph nodes tissues. Conclusion：The results suggest that the detection of K19 mRNA might be helpful to diagnose rectal cancer micrometastasis in lymph nodes. K19 mRNA detection by RT-PCR is superior to routine histpathological examination and is a highly sensitive and specific method.
出处
《临床肿瘤学杂志》
CAS
2008年第7期590-593,共4页
Chinese Clinical Oncology
基金
深圳市科技局立项课题(200603076)
关键词
直肠癌
逆转录聚合酶链反应
角蛋白19
淋巴结
微转移
Rectal cancer
Reverse transcriptase polymerase chain reaction （RT-PCR）
Keratin19 （ K19 ）
Lymph nodes
Micrometastasis
