人雄激素芳香化酶基因内含子中启动子的研究

The Studies of the Promoter in the Intron Ⅱ of the Human Aromatase Gene

利用核酸外切酶Ⅲ（ＥｘｏⅢ）对人雄激素芳香化酶基因的２４００ｂｐ片段的５’端进行系列缺失，并通过转染实验，分析了２４００ｂｐ片段３’端区域的功能作用，在雄激素芳香化酶基因第２外显子的下游区检测到１个具有启动子作用的功能元件，通过序列分析，发现该元件位于雄激素芳香化酶基因的第２内含子中．该启动子同样受到位于雄激素芳香化酶基因第１内含子中沉默因子的抑制作用．该启动子能够启动不同基因的表达，并且具有较强的启动功能．

In order to study the possible function of 2 400 bp fragment that contains partial sequence of human aromatase gene,DNA systematic deletion analysis of the fragment was performed,and a putative promoter in the fragment was found DNA sequence analysis revealed that the promoter was located in the second intron of thearomatase gene The function of the promoter was sappressed by a negative element,silencer,situated in the first intron The promoter can promote different gene expression in different vectors,but the degree of the expression is different from TK or mu MT promoter