摘要
建立了HPLC叔丁基对苯二酚含量的方法:色谱柱:Shim-pack Vp-ODS(150mm×4.6mm,5μm)接Cl8保护柱(20mm×4.6mm,5μm);以乙腈∶水=32∶68(V/V,含1%乙酸)作为流动相;流速:0.8mL/min;检测波长:290nm;柱温:25℃。结果表明TBHQ浓度在2×10-41.0mg/mL范围内与峰面积线性关系良好r≥0.9999,最低检测限为4ng,并建立了回收率及日内和日见精密度实验。
A sensitive and specific liquid chromatography method has been developed and validated for the determination of TBHQ. The chromatographic separation was achieved with a mixture of methanol and water （ V/V, 1% HAC） as mobile phase on a Shim-pack Vp-ODS column （150mm ×4.6 mm, 5um） with C18 column guard （20mm × 4.6mm, 5um）. The flow rate was 0. 8mL/min, and 290nm was set as determination wavelength. The method was proved to be accurate and precise at linearity ranges of 2 × 10^-4to 1.0mg/mL of TBHQ, respectively, with the correlation coefficient （r） ≥0. 9999. The lower limit of quantitation was 4 ng/mL. The intra-and inter-day precision and accuracy values were found to be within the accepted variable limits.
出处
《中国食品添加剂》
CAS
2008年第4期135-138,共4页
China Food Additives
