叶黄素对大鼠视网膜蓝光光损伤的保护作用

Protective effect of lutein against blue light-induced retinal damage in rat

目的观察叶黄素对大鼠视网膜蓝光损伤的保护作用。方法将大鼠随机分为正常对照组、模型对照组、溶剂对照组、叶黄素低、中、高剂量组。叶黄素低、中、高剂量组的浓度分别为0.5、1.0和2.0mg/ml,生理盐水和Tween-80以1∶9的比例配制成溶剂。叶黄素组及溶剂对照组大鼠玻璃体内分别注射不同剂量叶黄素及溶剂,注射量为5μl,暗适应24h,用蓝光损伤装置建立大鼠视网膜光损伤动物模型,光暴露时间为2h,光暴露后暗室饲养,72h后摘取眼球制备眼球壁石蜡切片,显微镜下观察视网膜形态学变化,测量外核层厚度,并计数外核层凋亡细胞。结果各剂量叶黄素组大鼠与模型对照组比,视网膜结构层次分明,细胞排列整齐;视网膜外核层厚度(40×10倍),正常对照组为(21.25±1.04)mm,模型对照组和溶剂对照组分别为(3.25±1.48)mm与(3.25±0.89)mm,叶黄素低、中、高剂量组分别为(15.00±5.58)mm、(11.75±4.20)mm及(14.75±3.96)mm,均显著高于模型对照组(P<0.01)。但该实验采用Tunel试剂盒检测细胞凋亡,各组间未见显著差别。结论在本实验条件下,叶黄素对大鼠视网膜光损伤有明显的保护作用。

Objective to investigate the effect of lutein on rat retina blue light damage. Methods Sprague-Dawley rat were randomly separated into 6 groups： normal control, model control, solvent control, low-dose, media-dose, and high-dose. The concentration of lutein solution in the low-dose, media-dose, and high-dose groups are 0.5mg/ml, 1.0mg/ ml,2.0mg/ml respectively. Mix sodium chloride and Tween 80 together at the ration of 1：9 as the solvent. Solvent and lutein solution were injected into rats＇ vitreous body of the solvent group and the lutein groups respectvely （the injection volume is 5μl）, dark adaptation 24h, then the rats exposed to the blue light equipment 2h to set up the light-damage animal model, After light exposure, the rats were raised in darkness for 72 hours. Then the rats were killed, the eyes were removed and were processed to paraffin section for microscopy, then we observed the changes of retina morphous, measured the thickness of the outer nuclear layer （ONL thickness）, and counted the number of apoptotic photoreceptors to compare the effect of lutein on light-damage of retina among different dosages. Results comparing with the model-control group, the rats of lutein group had more clearly demarcated retina structure and more ordered cells. After detected under microscopy, we found that the ONL thickness （40 ×10 times, mm） of the rats of normal control group was 21.25 ± 1.04. And the ONL thickness of the rats of lutein groups were 15.00 ± 5.58,11.75 ± 4.20 and 14.75 ± 3.96, from low dosage to high dosage, which was significantly （ P 〈 0.01 ） higher than those of the rats of model control group （ 3.25 ± 1.48 ） and solvent control group （3.25 ± 0.89 ）. Compare the number of apopotic photoreceptors, there is no significant differences among groups. Conclusion In the experiment conditions, the effect of prevention of lutein on retina light damage was significant. The result provided an important base on the application of lutein on crowd.