荧光光谱法结合多元曲线分辨-交替最小二乘法研究伞形花内酯与牛血清白蛋白的相互作用

Fluorescence Spectrometric Study on the Interaction Between Umbelliferone and Bovine Serum Albumin with the Use of MCR-ALS

在模拟人体生理条件下(pH=7.4),用荧光光谱法结合多元曲线分辨-交替最小二乘法(MCR-ALS)研究伞形花内酯与牛血清白蛋白(BSA)的相互作用.采用两种不同的试剂滴加模式对伞形花内酯与BSA的相互作用进行研究,对经典的荧光光谱数据矩阵加以扩展,增加了实验数据的信息量;进而应用MCR-ALS对该扩展的荧光光谱矩阵进行迭代计算,较好地分辨出作用过程中浓度变化趋势图,并计算出伞形花内酯与BSA的表观结合常数和结合比.通过同步荧光光谱法发现伞形花内酯对BSA的构象有一定的影响.

Under the imitated physiological conditions（ pH = 7.4）, the interaction between umbelliferone and bovine serum albumin（BSA） was investigated by the fluorescence spectroscopy and the multivariate curve res- olution-alternating least squares method （MCR-ALS）. In this work, the interaction between umbelliferone and bovine serum albumin（BSA） was studied by performing two different kinds of titration modes. The two spectroscopic data matrices were augmented, and then the information of experiment data was increased. The augmented experiment data matrix was analyzed by the MCR-ALS method. This procedure allowed the recovery of the concentration profiles for each＇ species and the calculation of the [BSA]： [Umbelliferone] ratio in the com- plex and the apparent equilibrium constant. The effect of umbelliferone on the conformation of BSA was also analyzed via synchronous fluoreseence spectrometry.