摘要
培养的胎牛主动脉内皮细胞具有可饱和性、高亲和性和配体持异性的LDL受体。该受体最大结合容量(Bmax)为378ng/mg细胞蛋白,平衡亲和常数(kd)为13μg/ml据估计,每个细胞表面的受体数目为8～9万。我们初步发现亚融合和融合但失去“接触抑制”特点的FBAE的LDL受体活性无明显差异。
The L DL receptors, which have such characteristics as saturability, high-affinity and ligand-specificity, are proved on cultured fetal bovine aortic endothelial cells. Its maximal binding capacity is 378ng/mg cell protein, kd is 13μg/ml and receptor numbers a cell are about 80-90 thousands. In addition, no obvious difference was also diseovered on L DL receptor activities on subconfluent and confluent , but not'contact inhibited' F B A Es.
