初乳对新生仔猪血液蛋白的电泳形态影响的研究

EFFECT OF COLOSTRUM ON SERUM PROTEINS OF NEWBORN PIGLETS

２４头太湖猪新生仔猪（二花×大白）分为四组，在出生后隔离并分别人工哺喂葡萄糖盐水（ＧＳ组，ｎ＝４），牛初乳（ＢＣ组，ｎ＝５），牛乳（ＢＭ组，ｎ＝６）４８ｈ和自然哺乳（ＰＣ组，ｎ＝９）。前３组在４８ｈ后回圈哺乳。初乳对新生仔猪血液蛋白电泳形态的影响，新生仔猪血清蛋白质浓度初生时平均为３１．８±１．８６ｍｇ／ｍｌ，ＰＣ组在出生后迅速增加，２４ｈ达８１．７８±８．６ｍｇ／ｍｌ此后略有下降。４８ｈ为５６．６７±１１．３６ｍｇ／ｍｌ，第７天为８７．６７±３．８９ｍｇ／ｍｌ。ＢＣ和ＢＭ组与ＰＣ组有相似的变化；而ＧＳ组在４８ｈ内一直下降，４８ｈ为６．２５±２．２８ｍｇ／ｍｌ，自然哺乳后，第７天上升为４７．７５±２．６８ｍｇ／ｍｌ。但ＢＭ、ＧＳ组在２４ｈ、４８ｈ和第７天血清总蛋白浓度显著低于ＰＣ组（Ｐ＜０．０１）。血清ＳＤＳ—ＰＡＧＥ电泳显示，清蛋白（ＳＡ）ＰＣ、ＢＣ、ＢＭ三组４８ｈ内持续增加，ＧＳ组２４ｈ后显著下降，并且ＧＳ组在高分子量区（１５０ｋｄ）出现非抗体蛋白和多肽的聚合，其他三组则未见到。实验中未观察到牛乳酪蛋白向仔猪血液中的转移。

おwentyfour newborn Taihui piglets were distributed into 4 groups and seperately fed by glucose saline (GS,n=4),bovine colostrum(BC,n=5),and bovine milk (BM,n=6)within 48 hours after birth,and then back to sowfold suckling freely,and the last group naturally suckled all the time (PC,n=9)Effect of colostrum on serum proteins of piglets was investigatedConcentrations of serum proteins of newborn piglets were at an average of 318±186mg/mlSerum protein of PC increased rapidly and reached 8178±86mg/ml at 24h ,and then slowly declined to 5667±1137mg/ml at 48hIt increased again to 8767±389mg/ml on day 7 The serum protein concentrations in BC and BM were changed in the similar way to that in PCSerum protein of GS kept decreasing within 48h(625±228mg/ml at 48h),but back to natural feedingIt was raised to 4775±268mg/ml on day 7The serum proteins in BM and GS were significantly lower than that in PC (P<001)at 24,48h and on day 7The band of serum albumin on SDS-PAGE gel showed that SA in PC,BC and BM constantly increased within 48h,But it declined after first 24h for GS,and some aggregations of serum proteins could be seen around 150kd on GS gelThere were no indications that bovine caseins of BC and BM were intactly absorbed into serum of piglets