摘要
马立克氏病毒是鸡的一种高度致肿瘤性疱疹病毒,主要以淋巴瘤为特征,有关该病毒的致病机理还不清楚。本研究利用澳大利亚分离的MDV致病毒株MPF57感染1日龄SPF雏鸡,并在感染后4周对其外周血淋巴细胞中的MDV特异性抗原进行了检测。结果表明,临床上呈急性感染并伴有明显症状的鸡的外周血淋巴细胞中的MDV抗原分子量主要为28、73、60和49kDa;而无明显症状的鸡的外周血淋巴细胞中的MDV抗原分子量主要为73kDa。试验证明,Westernblot是一种较好的检测病毒抗原的方法,可以检测到荧光抗体方法检测不到的淋巴细胞中的MDV特异抗原。
おareks disease (MD) is a lymphoproliferative disease of chickens caused by thehepersvirus, Mareks disease virus (MDV).It is unclear about the pathogenesis of MDV.In this report, we detected the viral antigens in the peripheral blood lymphocytes (PBL) of chickens infected with MPF57, isolate of MDV from Australia fields.The results showed that the molecular weights of MDV antigens were mainly 28 kDa,73 KDa,60kDa and 49 kDa in western bolt,which were from PBL with significant clinical symptoms.In comparison, 73 kDa antigen of MDV was from PBL without clinical symptoms. The antigens of MDV in PBL could be detected by western blot but may not be found in immunofluorescence assay. This results suggested that western blot be very useful in investigating the pathogenesis of MDV in the future.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第6期524-529,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
马立克氏病毒
抗原
淋巴细胞
免疫印迹
鸡病
Mareks disease virus,Antigens,Western blot,Peripheral blood lymphocytes