孕牛血中cAMP、cGMP水平变化的测定

The assay of blood cAMP and cGMP levels in pregnant dairy cows

选择３０头妊娠奶牛，根据妊娠不同分为妊娠前期（＜１００ｄ）、妊娠中期（１００～２００ｄ）和妊娠后期（＞２００ｄ）３组，应用放射免疫法测定孕牛血浆和外周血单个核细胞（ＰＢＭＣ）的ｃＡＭＰ和ｃＧＭＰ的含量．结果表明：随着妊娠期延长，孕牛血清和单个核细胞的ｃＡＭＰ是逐渐减少，而ｃＧＭＰ则是逐渐升高，但与对照组相比差异不显著（Ｐ＞０．０５）；血浆中ｃＡＭＰｃＧＭＰ之比值均比对照组高且差异显著（Ｐ＜０．０５），但随妊娠期延长而逐渐下降．

Thirty pregnant dairy cows were selected from a dairy herd and divided into 3 groups according to different gestation stages:viz. the first trimester (less than 100 days),the middle trimester (100 to 200 days) and the last trimester(more than 200 days). Radioimmunoassay was used to determine the plasma and mononuclear cell cAMP and cGMP levels in these pregnant dairy cows.The results showed that the longer the gestation duration, the lower the levels of cAMP,but the higher the levels of cGMP in plasma and mononuclear cells.The differences between 3 groups and non-pregnant dairy cows were not significant (P>0.05).The ratioe of plasma cAMP to cGMP in pregnant cows was obviously higher (P<0.[KG-4]05) than that in non-pregnant cows.However as the gestation duration increased, this ratio seemed to become lower.