流式细胞术微球阵列法检测急性白血病血清IL-6、IL-10和TNF水平

The levels of Il-6,IL-10,TNF detected by cytometric bead array during acute leukemia

目的:探讨流式细胞术微球阵列法(CBA)在细胞因子检测中的应用及血清肿瘤坏死因子(TNF)、白细胞介素6(IL-6)和白细胞介素10(IL-10)在不同亚型急性白血病(AL)中的表达水平。方法:采用BD CBA F lex Set IL-6、IL-10、TNF试剂盒和流式细胞术检测29例急性白血病患者和20例正常人血清样本中3种细胞因子水平。结果:AL患者IL-6、IL-10和TNF水平显著高于正常对照组,急性淋巴细胞白血病(ALL)的IL-10和TNF水平较急性非淋巴细胞白血病(ANLL)有显著性提高(P<0.05)。在ANLL亚型中,M1型IL-6水平最高。结论:CBA法能快速、准确地定量检测细胞因子;AL血清中IL-6、IL-10和TNF水平升高,可能与疾病的发生发展存在相关性。

Aim： To explore the application of cytometric bead array （CBA） method in detection of cytokines and detect the levels of IL-6,IL-10 and interferon （IFN） in the serum from patients with different subtypes of acute leukemia（AL）. Methods： The levels of three cytokines （IFN, IL-6, and IL-10） in the serum from 29 cases of patients with AL and 20 cases of normal persons were detected by using flow cytometry and BD CBA Flex Set kit. Results： The levels of IFN, IL-6, and IL-10 in AL group were significantly higher than those in controls. Compared with acute non-lymphoid leukemia （ANLL） group, the levels of IFN and IL-10 in acute lymphoid leukemia （ALL） group were significantly higher （P 〈0.05）. The level of IL-6 in M1 was the highest among the ANLL subtypes. Conclusion： Detecting cytokines by CBA method is quick and accurate; IFN, IL-6, and IL-10 might take part in the process of AL according to their increased levels in AL.